LOCUS       sequence-149869-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry (red) fluorophore with multiple cloning site for use with 
            inducible N-terminal fusions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: p3E-mCherry-NS-MCS
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(35..158)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      161..170
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     885..901
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     886..902
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(942..962)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ctacaggtca ctaataccat ctaagtagtt ggttcatagt gactgcatat gttgtgtttt
       61 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
      121 attttacgtt tctcgttcaa ctttcttgta caaagtggct gccaccatgg tgtccaaggg
      181 cgaagaggac aacatggcca tcatcaaaga gttcatgcgg ttcaaggtgc acatggaagg
      241 cagcgtgaac ggccacgagt tcgagattga gggcgagggc gaaggcagac cctacgaggg
      301 aacacagacc gccaagctga aagtgaccaa gggcggaccc ctgcccttcg cctgggatat
      361 tctgagcccc cagtttatgt acggcagcaa ggcctacgtg aagcaccccg ccgacatccc
      421 cgactacctg aagctgagct tccccgaggg cttcaagtgg gagagagtga tgaacttcga
      481 ggacggcggc gtcgtgaccg tgacccagga tagctctctg caggacggcg agttcatcta
      541 caaagtgaag ctgcggggca ccaacttccc cagcgacgga cccgtgatgc agaaaaagac
      601 catgggctgg gaggccagca gcgagagaat gtaccctgag gatggcgccc tgaagggcga
      661 gatcaagcag cggctgaaac tgaaggatgg cggccactac gacgccgaag tgaaaaccac
      721 ctacaaggcc aagaaacccg tgcagctgcc tggcgcctac aacgtgaaca tcaagctgga
      781 catcaccagc cacaacgagg actacaccat cgtggaacag tacgagcggg ccgagggcag
      841 acactctaca ggcggaatgg acgagctgta caagcacgga tccctcgagg tcgacggtat
      901 cgataagctt gatatcgaat tccccgggtc tagaactagt gcaactttat tatacaaagt
      961 tggcatta
//
