LOCUS       sequence-149877-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 
            (orange) fluorophore separated by a self-cleaving 2A peptide for use
            with C-terminal fusions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(39..162)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(880..899)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aattctacag gtcactaata ccatctaagt agttggttca tagtgactgc atatgttgtg
       61 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
      121 tatcatttta cgtttctcgt tcaactttct tgtacaaagt gggaattcgc cgccaccatg
      181 cctgaaccct ctaagaaggg aagcaagaag gccatcacca aggcccagaa gaaggacgga
      241 aagaagcgca agagaagccg caaagagtct tacagcatct acgtgtacaa ggtgctgaaa
      301 caggtgcacc ctgacaccgg aatcagcagc aaggccatgg gaatcatgaa cagcttcgtg
      361 aacgacatct tcgagagaat cgccggcgag gccagcagac tggcccacta caacaagaga
      421 agcaccatca ccagccgcga gatccagacc gccgtcagac tgctgctgcc tggcgagctg
      481 gccaagcacg ccgtgtctga gggaacaaag gccgtgacca agtacacaag cagcaagggc
      541 ggaggaagcg gaggcggaag cggcggagga tctatggtgt ctaagggcga ggaactgttc
      601 accggcgtgg tgcctatcct ggtggaactg gacggcgacg tgaacggaca caagttcagc
      661 gtgtccggcg agggcgaagg cgacgccacc tacggaaagc tgaccctgaa gttcatctgc
      721 accaccggaa agctgcctgt gccttggcct accctggtca ccaccctgag ctggggagtg
      781 cagtgcttcg ccagataccc tgaccacatg aagcagcacg atttcttcaa gagcgccatg
      841 cctgagggat acgtgcagga aagaaccatc ttcttcaagg acgacggcaa ctacaagacc
      901 agagccgaag tgaagttcga gggcgacacc ctggtcaaca gaatcgagct gaagggaatc
      961 gacttcaaag aggacggaaa catcctggga cacaagctgg agtacaacgc catcc
//
