LOCUS       sequence-149878-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 
            (orange) fluorophore separated by a self-cleaving 2A peptide for use
            with C-terminal fusions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    31..126
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(196..849)
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             complement(856..912)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gcggccgcgt taacgctacc atggagctcc aaataatgat tttattttga ctgatagtga
       61 cctgttcgtt gcaacaaatt gataagcaat gcttttttat aatgccaact ttgtataata
      121 aagttgacta gttcagctca gcacgcattt gcagctcatg catccaggtc cgctctcgtc
      181 tggagggttc agcttgctgt gagccacggc gtcctcgacc tgctcggtga tgtttccctc
      241 ggtcttgcgg accagtctgt gtccgatgta gtggtcgcct ggcatttcca ggatttcttt
      301 ggcggccttg taggtggtct tcatctggca cttgtggttg cctccgcctt ccagcttcag
      361 gtacatggtc acgtcgccct tcagcacgcc gtcgctggcg gtgatcttct cggtgctagg
      421 ctcccagtcc acgctctggt tctgcatgat aggtccgtcg gcagggaagt tcacgcctgt
      481 gaacttgctc ttgtggtaga aggtgtttcc tctcaggctg atgtgggcgg acacgctagc
      541 gcttccgccg tcctcaaatt ccaggcttct ctcccagctc agtccctcag ggaaggcctg
      601 cttgaagtag tcggggattt cctcagggta cttggtgaac actctgtgtc cgtagcagaa
      661 cacgtgagac accaggtcga aggcgaaagg cataggtccg ccctcggcca tggtgactct
      721 cagggtcatt tcctggtgtc cctcgtaagg tctgccggtt ccctcgccct cgatggtaaa
      781 ctcgtggccg ttcacagatc cgtccatgta gtagcgcatc ttcatctcag gcttgatcac
      841 agacaccatc ccgggaggtc cagggttctc ttccacgtcg ccggcctgct tcagcaggct
      901 gaagttggtg gctccgcttc ccctaggttt atacagttca tccatgccca gggtgattcc
      961 agcggcggtc acaaattcca gcagcaccat gtggtcgcgc ttctcgttag ggt
//
