LOCUS       sequence-149880-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3b2-FL3
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..71
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             75..122
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     terminator      152..339
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(207..231)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        398..741
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 acgctgcagg tcgacggatc cccgggttaa ttaacgctag catgggtgac tacaaggacg
       61 acgatgacaa gggagattac aaagatgacg acgataagga ctacaaggac gacgatgaca
      121 agtctagatg aggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat
      181 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
      241 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
      301 atagtatgag gtcgctctta ttgaccacac ctctaccggc agatccgcta ggagatctgt
      361 ttagcttgcc tcgtccccgc cgggtcaccc ggtcagcgac atggaggccc agaataccct
      421 ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta
      481 gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg
      541 aagcaaaaat tacggctcct cgctgcggac ctgcgagcag ggaaacgctc ccctcacaga
      601 cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc
      661 actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat
      721 cacatccgaa cataaacaac catgggtaaa aagcctgaac tcaccgcgac gtctgtcgag
      781 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
      841 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
      901 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
      961 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     1021 cgccgtgcac aggg
//
