LOCUS       sequence-149881-        8904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY destination vector for site-specific integration into the 
            human AAVS1/PPP1R12C locus for doxycycline-inducible gene 
            over-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D
  TITLE     Genome Editing of Lineage Determinants in Human Pluripotent Stem 
            Cells Reveals Mechanisms of Pancreatic Development and Diabetes.
  JOURNAL   Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015.
  PUBMED    27133796
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015."
COMMENT     Sequence Label: Puro-iDEST
FEATURES             Location/Qualifiers
     source          1..8904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..804
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    811..836
                     /label=SA
                     /note="splice acceptor site"
     CDS             860..913
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             923..1522
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(923..942)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1419..1439
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1554..1571)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1560..1784
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    1791..1872
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1816..1835
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    2056..2180
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2221..2526)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(2868..3527)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3442..3461
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(3581..3587,3588..3605,3606..3611))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(3636..3760)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(3777..4091)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(3780..3804)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3851..3869
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3887..3905
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3922..3940
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3958..3976
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3994..4012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4029..4047
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4065..4083
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    4108..4944
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(4978..4997)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4979..4997)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5004..5021)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5004..5020)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5013..5035)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5158..5460
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(5863..5882)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6477..6496
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(6857..7648,7649..7717))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7480..7499
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7841..8429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8330..8349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8583..8600
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8717..8738
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8753..8758,8759..8776,8777..8783)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8791..8807
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8796..8818
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8815..8831
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8815..8831
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8852..8870
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgctttctct gaccagcatt ctctcccctg ggcctgtgcc gctttctgtc tgcagcttgt
       61 ggcctgggtc acctctacgg ctggcccaga tccttccctg ccgcctcctt caggttccgt
      121 cttcctccac tccctcttcc ccttgctctc tgctgtgttg ctgcccaagg atgctctttc
      181 cggagcactt ccttctcggc gctgcaccac gtgatgtcct ctgagcggat cctccccgtg
      241 tctgggtcct ctccgggcat ctctcctccc tcacccaacc ccatgccgtc ttcactcgct
      301 gggttccctt ttccttctcc ttctggggcc tgtgccatct ctcgtttctt aggatggcct
      361 tctccgacgg atgtctccct tgcgtcccgc ctccccttct tgtaggcctg catcatcacc
      421 gtttttctgg acaaccccaa agtaccccgt ctccctggct ttagccacct ctccatcctc
      481 ttgctttctt tgcctggaca ccccgttctc ctgtggattc gggtcacctc tcactccttt
      541 catttgggca gctcccctac cccccttacc tctctagtct gtgctagctc ttccagcccc
      601 ctgtcatggc atcttccagg ggtccgagag ctcagctagt cttcttcctc caacccgggc
      661 ccctatgtcc acttcaggac agcatgtttg ctgcctccag ggatcctgtg tccccgagct
      721 gggaccacct tatattccca gggccggtta atgtggctct ggttctgggt acttttatct
      781 gtcccctcca ccccacagtg gggcaagctt ctgacctctt ctcttcctcc cacagggcct
      841 cgagagatct ggcagcggag agggcagagg aagtcttcta acatgcggtg acgtggagga
      901 gaatcccggc cctaggctcg agatgaccga gtacaagccc acggtgcgcc tcgccacccg
      961 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     1021 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     1081 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     1141 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     1201 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     1261 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     1321 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     1381 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     1441 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     1501 gacccgcaag cccggtgcct gatctagagg gcccgtttaa acccgctgat cagcctcgac
     1561 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     1621 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     1681 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     1741 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atgggtcgag gcagtgaaaa
     1801 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     1861 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     1921 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct
     1981 ctagagatat cgtcgacaag cttatcgatg cggccgcgct agcacgcgtc agctgactag
     2041 aggatccccg gatcaaccac tttgtacaag aaagctgaac gagaaacgta aaatgatata
     2101 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
     2161 caacatatcc agtcactatg gtcgacctgc agactggctg tgtataaggg agcctgacat
     2221 ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag
     2281 atcagccact tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat
     2341 gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga
     2401 cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc
     2461 actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa
     2521 ctgcatttca ccagcccctg ttctcgtcag caaaagagcc gttcatttca ataaaccggg
     2581 cgacctcagc catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg
     2641 cttcattctg catggttgtg cttaccagac cggagatatt gacatcatat atgccttgag
     2701 caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag cacacctctt
     2761 tttgacatac ttcgggtata catatcagta tatattctta taccgcaaaa atcagcgcgc
     2821 aaatacgcat actgttatct ggcttttagt aagccggatc cagatcttta cgccccgccc
     2881 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     2941 cagacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     3001 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     3061 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     3121 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     3181 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     3241 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     3301 gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     3361 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     3421 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     3481 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     3541 gctcctgaaa atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca
     3601 taaagtgtaa agcctggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt
     3661 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
     3721 cattttacgt ttctcgttca gcttttttgt acaaacttgt tgatcggaat tctccaggcg
     3781 atctgacggt tcactaaacg agctctgctt atataggcct cccaccgtac acgcctacct
     3841 cgacatacgt tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat
     3901 agggataaac tcgacatacg ttctctatca ctgataggga gtaaactcga catacgttct
     3961 ctatcactga tagggagtaa actcgacata cgttctctat cactgatagg gagtaaactc
     4021 gacatcgttc tctatcactg atagggagta aactcgacat acgttctcta tcactgatag
     4081 ggagtaaact cgacagtact aagctttact agggacagga ttggtgacag aaaagcccca
     4141 tccttaggcc tcctccttcc tagtctcctg atattgggtc taacccccac ctcctgttag
     4201 gcagattcct tatctggtga cacaccccca tttcctggag ccatctctct ccttgccaga
     4261 acctctaagg tttgcttacg atggagccag agaggatcct gggagggaga gcttggcagg
     4321 gggtgggagg gaaggggggg atgcgtgacc tgcccggttc tcagtggcca ccctgcgcta
     4381 ccctctccca gaacctgagc tgctctgacg cggctgtctg gtgcgtttca ctgatcctgg
     4441 tgctgcagct tccttacact tcccaagagg agaagcagtt tggaaaaaca aaatcagaat
     4501 aagttggtcc tgagttctaa ctttggctct tcacctttct agtccccaat ttatattgtt
     4561 cctccgtgcg tcagttttac ctgtgagata aggccagtag ccagccccgt cctggcaggg
     4621 ctgtggtgag gaggggggtg tccgtgtgga aaactccctt tgtgagaatg gtgcgtccta
     4681 ggtgttcacc aggtcgtggc cgcctctact ccctttctct ttctccatcc ttctttcctt
     4741 aaagagtccc cagtgctatc tgggacatat tcctccgccc agagcagggt cccgcttccc
     4801 taaggccctg ctctgggctt ctgggtttga gtccttggca agcccaggag aggcgctcag
     4861 gcttccctgt cccccttcct cgtccaccat ctcatgcccc tggctctcct gccccttccc
     4921 tacaggggtt cctggctctg ctctaagggc gaattcgcgg ccgctaaatt caattcgccc
     4981 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5041 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     5101 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctata cgtacggcag
     5161 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt
     5221 gatattattg acacgccggg gcgacggatg gtgatccccc tggccagtgc acgtctgctg
     5281 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc
     5341 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat
     5401 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
     5461 atgtcaggca tgagattatc aaaaaggatc ttcacctaga tccttttcac gtagaaagcc
     5521 agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg
     5581 gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta
     5641 gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt
     5701 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg
     5761 cgcaggggat caagctctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     5821 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     5881 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     5941 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     6001 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     6061 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     6121 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     6181 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     6241 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     6301 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     6361 gtcgtgaccc atgcatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     6421 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     6481 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     6541 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     6601 cttctgaatt attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     6661 cggtatttca caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6721 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagattatca aaaaggatct
     6781 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6841 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6901 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6961 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7021 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7081 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7141 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7201 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7261 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7321 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7381 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7441 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7501 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7561 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7621 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7681 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7741 gaagcattta tcagggttat tgtctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7981 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8521 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8581 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8641 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8701 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     8761 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     8821 acagctatga ccatgattac gccaagctca gaattaaccc tcactaaagg gactagtcct
     8881 gcaggtttaa acgaattcgc cctt
//
