LOCUS       sequence-149889-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3b2-PK3
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..81
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             85..126
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             127..168
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      198..385
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(253..277)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        444..787
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aacgctagcg gtaagcctat tcctaaccct
       61 cttttgggtc tcgattctac aggtggaaaa ccaatcccca acccactcct cggccttgac
      121 tcaactggca aacccatacc aaatccttta cttggattag atagtacctc tagatgaggc
      181 gcgccacttc taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta
      241 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct
      301 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag tatgaggtcg
      361 ctcttattga ccacacctct accggcagat ccgctaggag atctgtttag cttgcctcgt
      421 ccccgccggg tcacccggtc agcgacatgg aggcccagaa taccctcctt gacagtcttg
      481 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc
      541 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg
      601 gctcctcgct gcggacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc
      661 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct
      721 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata
      781 aacaaccatg ggtaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
      841 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
      901 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
      961 ctacaaagat cgttatgttt atcggcactt tgca
//
