LOCUS       sequence-149894-        1710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Cx32-7
FEATURES             Location/Qualifiers
     source          1..1710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      952..961
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             958..1668
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gatctcgagc tcaagcttcg aattccctct gggaaagggc agcagcagcc aggtgtggca
       61 gtgacaggga ggtgtgaatg aggcaggatg aactggacag gtttgtacac cttgctcagt
      121 ggcgtgaacc ggcattctac tgccattggc cgagtatggc tctcggtcat cttcatcttc
      181 agaatcatgg tgctggtggt ggctgcagag agtgtgtggg gtgatgagaa atcttccttc
      241 atctgcaaca cactccagcc tggctgcaac agcgtttgct atgaccaatt cttccccatc
      301 tcccatgtgc ggctgtggtc cctgcagctc atcctagttt ccaccccagc tctcctcgtg
      361 gccatgcacg tggctcacca gcaacacata gagaagaaaa tgctacggct tgagggccat
      421 ggggaccccc tacacctgga ggaggtgaag aggcacaagg tccacatctc agggacactg
      481 tggtggacct atgtcatcag cgtggtgttc cggctgttgt ttgaggccgt cttcatgtat
      541 gtcttttatc tgctctaccc tggctatgcc atggtgcggc tggtcaagtg cgacgtctac
      601 ccctgcccca acacagtgga ctgcttcgtg tcccgcccca ccgagaaaac cgtcttcacc
      661 gtcttcatgc tagctgcctc tggcatctgc atcatcctca atgtggccga ggtggtgtac
      721 ctcatcatcc gggcctgtgc ccgccgagcc cagcgccgct ccaatccacc ttcccgcaag
      781 ggctcgggct tcggccaccg cctctcacct gaatacaagc agaatgagat caacaagctg
      841 ctgagtgagc aggatggctc cctgaaagac atactgcgcc gcagccctgg caccggggct
      901 gggctggctg aaaagagcga ccgctgctcg gcctgcgcgg atccaccggt cgccaccatg
      961 gtgtctaagg gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc
     1021 accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     1081 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     1141 ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc
     1201 gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     1261 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     1321 aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca
     1381 ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga
     1441 aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacgc caagaccaca
     1501 tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac
     1561 agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca
     1621 gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatta agcggccgcg
     1681 actctagatc ataatcagcc ataccacatt
//
