LOCUS       sequence-149896-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3b-PK3
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..85
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             89..130
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             131..172
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      202..389
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(257..281)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        448..791
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gtacgctgca ggtcgacgga tccccgggtt aattaacgct agcggtaagc ctattcctaa
       61 ccctcttttg ggtctcgatt ctacaggtgg aaaaccaatc cccaacccac tcctcggcct
      121 tgactcaact ggcaaaccca taccaaatcc tttacttgga ttagatagta cctctagatg
      181 aggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa
      241 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
      301 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag
      361 gtcgctctta ttgaccacac ctctaccggc agatccgcta ggagatctgt ttagcttgcc
      421 tcgtccccgc cgggtcaccc ggtcagcgac atggaggccc agaataccct ccttgacagt
      481 cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat
      541 ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat
      601 tacggctcct cgctgcggac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat
      661 tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc
      721 ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa
      781 cataaacaac catgggtaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
      841 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
      901 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
      961 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     1021 aagtgcttga cattggggaa ttcagcg
//
