LOCUS       sequence-149897-        7948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline inducible Ngn3 over-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D
  TITLE     Genome Editing of Lineage Determinants in Human Pluripotent Stem 
            Cells Reveals Mechanisms of Pancreatic Development and Diabetes.
  JOURNAL   Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015.
  PUBMED    27133796
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015."
COMMENT     Sequence Label: Puro-iNgn3
FEATURES             Location/Qualifiers
     source          1..7948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    809..834
                     /label=SA
                     /note="splice acceptor site"
     CDS             858..911
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             921..1520
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(921..940)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1417..1437
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1552..1569)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1558..1782
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    1789..1870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1814..1833
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    2054..2078
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2778..2802)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2819..3133)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(2822..2846)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2893..2911
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2929..2947
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2964..2982
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3000..3018
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3036..3054
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3071..3089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3107..3125
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    3150..3986
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(4020..4039)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4021..4039)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4046..4063)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4046..4062)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4055..4077)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             4200..4502
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4381..4400
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(4905..4924)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5519..5538
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(5899..6690,6691..6759))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6522..6541
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6883..7471
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7372..7391
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7625..7642
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7759..7780
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7795..7800,7801..7818,7819..7825)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7833..7849
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7838..7860
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7857..7873
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7857..7873
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7894..7912
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(7947..7948,1..802)
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 ctttctctga ccagcattct ctcccctggg cctgtgccgc tttctgtctg cagcttgtgg
       61 cctgggtcac ctctacggct ggcccagatc cttccctgcc gcctccttca ggttccgtct
      121 tcctccactc cctcttcccc ttgctctctg ctgtgttgct gcccaaggat gctctttccg
      181 gagcacttcc ttctcggcgc tgcaccacgt gatgtcctct gagcggatcc tccccgtgtc
      241 tgggtcctct ccgggcatct ctcctccctc acccaacccc atgccgtctt cactcgctgg
      301 gttccctttt ccttctcctt ctggggcctg tgccatctct cgtttcttag gatggccttc
      361 tccgacggat gtctcccttg cgtcccgcct ccccttcttg taggcctgca tcatcaccgt
      421 ttttctggac aaccccaaag taccccgtct ccctggcttt agccacctct ccatcctctt
      481 gctttctttg cctggacacc ccgttctcct gtggattcgg gtcacctctc actcctttca
      541 tttgggcagc tcccctaccc cccttacctc tctagtctgt gctagctctt ccagccccct
      601 gtcatggcat cttccagggg tccgagagct cagctagtct tcttcctcca acccgggccc
      661 ctatgtccac ttcaggacag catgtttgct gcctccaggg atcctgtgtc cccgagctgg
      721 gaccacctta tattcccagg gccggttaat gtggctctgg ttctgggtac ttttatctgt
      781 cccctccacc ccacagtggg gcaagcttct gacctcttct cttcctccca cagggcctcg
      841 agagatctgg cagcggagag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
      901 atcccggccc taggctcgag atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
      961 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     1021 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     1081 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     1141 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     1201 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     1261 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     1321 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     1381 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     1441 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     1501 cccgcaagcc cggtgcctga tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     1561 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     1621 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     1681 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     1741 aagacaatag caggcatgct ggggatgcgg tgggctctat gggtcgaggc agtgaaaaaa
     1801 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     1861 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     1921 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     1981 agagatatcg tcgacaagct tatcgatgcg gccgcgctag cacgcgtcag ctgactagag
     2041 gatccccgga tcaaccactt tgtacaagaa agctgggtct agatatctcg agtgcggccg
     2101 ctcacaagaa gtctgagaac accagtgctc ccgggagcag ataggatggg gagctgggca
     2161 cctgcaggcc agggaattcc tccaatgagg ccgtggggct caggttaccc gcttgggaga
     2221 ctggggagta gatagagccc cagtccccgt tggagccacc tccggggctc cccagctctc
     2281 cacagggcac agggggctcc gggccataga agctgtggtc cgctatgcgc agcgtctgag
     2341 tcagtgccca gatgtagttg tgggcgaagc gcagggtctc gatctttgta agtttggcgt
     2401 catccgggaa ggtgggcagg acaccgcgca gcgcatccag cgccgagttg aggttgtgca
     2461 tgcgattgcg ctcccgatca ttggccttct tgcgccggct tcttcgctgt ttgctgagtg
     2521 ccaactcgct cttgggcctg ttgcgccctc cgcgtcgggc gcggagcttc ctcgaggtcc
     2581 ctcggcagtc acccacttct gcttcggagc agtccctagg tatgagagtg gggctaggtg
     2641 gggtggaatt ggaactgagc acttcgtggt ccgaggctcc gggaaaaggt tgttgtgtct
     2701 ctggggacac ttggatggtg agcgcatcca agggatgagg cgccatggat ccagtcgact
     2761 gaattggttc cggcgccagc ctgctttttt gtacaaactt gttgatcgga attctccagg
     2821 cgatctgacg gttcactaaa cgagctctgc ttatataggc ctcccaccgt acacgcctac
     2881 ctcgacatac gttctctatc actgataggg agtaaactcg acatacgttc tctatcactg
     2941 atagggataa actcgacata cgttctctat cactgatagg gagtaaactc gacatacgtt
     3001 ctctatcact gatagggagt aaactcgaca tacgttctct atcactgata gggagtaaac
     3061 tcgacatcgt tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat
     3121 agggagtaaa ctcgacagta ctaagcttta ctagggacag gattggtgac agaaaagccc
     3181 catccttagg cctcctcctt cctagtctcc tgatattggg tctaaccccc acctcctgtt
     3241 aggcagattc cttatctggt gacacacccc catttcctgg agccatctct ctccttgcca
     3301 gaacctctaa ggtttgctta cgatggagcc agagaggatc ctgggaggga gagcttggca
     3361 gggggtggga gggaaggggg ggatgcgtga cctgcccggt tctcagtggc caccctgcgc
     3421 taccctctcc cagaacctga gctgctctga cgcggctgtc tggtgcgttt cactgatcct
     3481 ggtgctgcag cttccttaca cttcccaaga ggagaagcag tttggaaaaa caaaatcaga
     3541 ataagttggt cctgagttct aactttggct cttcaccttt ctagtcccca atttatattg
     3601 ttcctccgtg cgtcagtttt acctgtgaga taaggccagt agccagcccc gtcctggcag
     3661 ggctgtggtg aggagggggg tgtccgtgtg gaaaactccc tttgtgagaa tggtgcgtcc
     3721 taggtgttca ccaggtcgtg gccgcctcta ctccctttct ctttctccat ccttctttcc
     3781 ttaaagagtc cccagtgcta tctgggacat attcctccgc ccagagcagg gtcccgcttc
     3841 cctaaggccc tgctctgggc ttctgggttt gagtccttgg caagcccagg agaggcgctc
     3901 aggcttccct gtcccccttc ctcgtccacc atctcatgcc cctggctctc ctgccccttc
     3961 cctacagggg ttcctggctc tgctctaagg gcgaattcgc ggccgctaaa ttcaattcgc
     4021 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     4081 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     4141 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagccta tacgtacggc
     4201 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     4261 gtgatattat tgacacgccg gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     4321 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     4381 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     4441 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     4501 aaatgtcagg catgagatta tcaaaaagga tcttcaccta gatccttttc acgtagaaag
     4561 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa
     4621 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
     4681 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
     4741 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
     4801 ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4861 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4921 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4981 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     5041 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5101 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5161 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5221 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5281 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5341 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     5401 tcgtcgtgac ccatgcatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     5461 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5521 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5581 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5641 ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg
     5701 tgcggtattt cacaccgcat caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5761 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagattat caaaaaggat
     5821 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5881 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5941 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6001 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6061 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6121 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6181 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6241 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6301 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6361 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6421 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6481 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6541 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6601 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6661 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6721 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6781 ttgaagcatt tatcagggtt attgtctcat gaccaaaatc ccttaacgtg agttttcgtt
     6841 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6901 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6961 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7021 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7081 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7141 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7201 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7261 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7321 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7381 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7441 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7501 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7561 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7621 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     7681 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     7741 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     7801 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     7861 aaacagctat gaccatgatt acgccaagct cagaattaac cctcactaaa gggactagtc
     7921 ctgcaggttt aaacgaattc gccctttg
//
