LOCUS       sequence-149898-        9037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline inducible Notch1 intracellular domain gene 
            over-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D
  TITLE     Genome Editing of Lineage Determinants in Human Pluripotent Stem 
            Cells Reveals Mechanisms of Pancreatic Development and Diabetes.
  JOURNAL   Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015.
  PUBMED    27133796
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 
            10.1016/j.stem.2016.03.015."
COMMENT     Sequence Label: Puro-iNotch1IC
FEATURES             Location/Qualifiers
     source          1..9037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    809..834
                     /label=SA
                     /note="splice acceptor site"
     CDS             858..911
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             921..1520
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(921..940)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1417..1437
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1552..1569)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1558..1782
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    1789..1870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1814..1833
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    2054..2078
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3764..3787)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(3867..3891)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3908..4222)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(3911..3935)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3982..4000
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4018..4036
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4053..4071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4089..4107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4125..4143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4160..4178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4196..4214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    4239..5075
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(5109..5128)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5110..5128)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5135..5152)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5135..5151)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5144..5166)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5289..5591
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5470..5489
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(5994..6013)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6608..6627
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(6988..7779,7780..7848))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7611..7630
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7972..8560
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8461..8480
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8714..8731
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8848..8869
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8884..8889,8890..8907,8908..8914)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8922..8938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8927..8949
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8946..8962
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8946..8962
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8983..9001
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(9036..9037,1..802)
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 ctttctctga ccagcattct ctcccctggg cctgtgccgc tttctgtctg cagcttgtgg
       61 cctgggtcac ctctacggct ggcccagatc cttccctgcc gcctccttca ggttccgtct
      121 tcctccactc cctcttcccc ttgctctctg ctgtgttgct gcccaaggat gctctttccg
      181 gagcacttcc ttctcggcgc tgcaccacgt gatgtcctct gagcggatcc tccccgtgtc
      241 tgggtcctct ccgggcatct ctcctccctc acccaacccc atgccgtctt cactcgctgg
      301 gttccctttt ccttctcctt ctggggcctg tgccatctct cgtttcttag gatggccttc
      361 tccgacggat gtctcccttg cgtcccgcct ccccttcttg taggcctgca tcatcaccgt
      421 ttttctggac aaccccaaag taccccgtct ccctggcttt agccacctct ccatcctctt
      481 gctttctttg cctggacacc ccgttctcct gtggattcgg gtcacctctc actcctttca
      541 tttgggcagc tcccctaccc cccttacctc tctagtctgt gctagctctt ccagccccct
      601 gtcatggcat cttccagggg tccgagagct cagctagtct tcttcctcca acccgggccc
      661 ctatgtccac ttcaggacag catgtttgct gcctccaggg atcctgtgtc cccgagctgg
      721 gaccacctta tattcccagg gccggttaat gtggctctgg ttctgggtac ttttatctgt
      781 cccctccacc ccacagtggg gcaagcttct gacctcttct cttcctccca cagggcctcg
      841 agagatctgg cagcggagag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
      901 atcccggccc taggctcgag atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
      961 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     1021 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     1081 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     1141 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     1201 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     1261 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     1321 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     1381 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     1441 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     1501 cccgcaagcc cggtgcctga tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     1561 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     1621 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     1681 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     1741 aagacaatag caggcatgct ggggatgcgg tgggctctat gggtcgaggc agtgaaaaaa
     1801 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     1861 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     1921 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     1981 agagatatcg tcgacaagct tatcgatgcg gccgcgctag cacgcgtcag ctgactagag
     2041 gatccccgga tcaaccactt tgtacaagaa agctgggtct agatatctcg agtgcggccg
     2101 cgaattcgat aattctcgag ctagctacct agcttgccgg tgcacccacg gtgaaattca
     2161 tagcccccgt gccattggta ctcagcactg tgctggcact ggaggctaca ggcaggtggg
     2221 agaggcgtgg cgggggtggc tcaaaggcca accggctacc tccagccagt gctgccatct
     2281 caggcttggc tgccacattc aagtggctga tgcccaggtg agtgtcaggc ataccaggca
     2341 ggtggctgag aggcatggat ggagactgct ggaatgggga ggggaggagg ggtggcgagg
     2401 ccacatctga caagtagcca tggggtgact cgagggagtc cacaggcgac agcatgctcg
     2461 agctgtccaa caggcagccc ttgccatcct gggacttctt cctccgtgcc ttgaggtcct
     2521 tagcttcctt gctaccacaa gccagccctt tggtgctggg cttgcgggcc ttcttgccct
     2581 gtgtggcaga cttgagattg cccaggtagc cattgggcga gcagagtgtg ggagacagag
     2641 tgggtgtgcc acccagggca gtgccatgca gctgtgggct gcgcaccagg ttgtactcat
     2701 ccaaaagccg cacgatatcg tggtgcatac gctcctgtgc gatgtcccgc ggcaatcggt
     2761 ccatgtgatc cgtgatgtcc cggttggcaa agtggtccag caacactttg gcagtctcat
     2821 agctgccctc acgggcggcc aggaacaggg gagtctcctc cttgttgttc tgcatgtcct
     2881 tgttggctcc gttcttcagg agcacaacag cagcatccac attgttcacc gcggccgccc
     2941 aatgcaaagc cgacttgcct aggtcatcca cggcattgac gtcagcatgt gagttgatga
     3001 ggtcctccag catgccctcc agggccaggc gcgcagccag gatcagtgga gttgtgccat
     3061 catgcattcg ggcatccaga tctgtggccc tgttccggag caggatctgg aagacaccct
     3121 gagcatctgc agaaactgct gcatgtaacg gagtacggcc catgttgtcc tggatgttgg
     3181 catctgcact ggcctccagc aagcgctttg cagcatctga acgagagtat cgggcagcca
     3241 agtgcaaggc ggtctccccg gtgcggtctg tctggttgtg caagctggcg ccctggtaga
     3301 tgaagtcaga gatgacagca ggtgcatctt cttcttcttc actgttgcct gtctcaaggc
     3361 cccctccact gcaggaggca atcatgaggg gtgtgaagcc atctggtcct cgaacattga
     3421 catccatgca gtcagcatcc acctccccct gaggcggtgt tggggccatg gcagacatgc
     3481 gcaggtcagc agcgtccagg tgctgctggg tccactgtct gtggtcagtc tgatcactca
     3541 ggtcagggag aactactggc tcctcaaacc ggaacttctt ggtctccagg tcttcgtctc
     3601 cccactcgtt ctgattgtcg tccatcagag caccatctga ggcattcttc aggggcttga
     3661 ggccgactga gtcctcgccg aggggctctc tccgcttctt cttgctggcc tctgacactt
     3721 tgaaaccctc agggaaccag agctggccat gctgccgccg ggctttatcg tcatcgtctt
     3781 tgtagtccat ggtaccgaat tgggccggct agctagctag aattatcaag cttatcgata
     3841 ccgtcgactg aattggttcc ggcgccagcc tgcttttttg tacaaacttg ttgatcggaa
     3901 ttctccaggc gatctgacgg ttcactaaac gagctctgct tatataggcc tcccaccgta
     3961 cacgcctacc tcgacatacg ttctctatca ctgataggga gtaaactcga catacgttct
     4021 ctatcactga tagggataaa ctcgacatac gttctctatc actgataggg agtaaactcg
     4081 acatacgttc tctatcactg atagggagta aactcgacat acgttctcta tcactgatag
     4141 ggagtaaact cgacatcgtt ctctatcact gatagggagt aaactcgaca tacgttctct
     4201 atcactgata gggagtaaac tcgacagtac taagctttac tagggacagg attggtgaca
     4261 gaaaagcccc atccttaggc ctcctccttc ctagtctcct gatattgggt ctaaccccca
     4321 cctcctgtta ggcagattcc ttatctggtg acacaccccc atttcctgga gccatctctc
     4381 tccttgccag aacctctaag gtttgcttac gatggagcca gagaggatcc tgggagggag
     4441 agcttggcag ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc
     4501 accctgcgct accctctccc agaacctgag ctgctctgac gcggctgtct ggtgcgtttc
     4561 actgatcctg gtgctgcagc ttccttacac ttcccaagag gagaagcagt ttggaaaaac
     4621 aaaatcagaa taagttggtc ctgagttcta actttggctc ttcacctttc tagtccccaa
     4681 tttatattgt tcctccgtgc gtcagtttta cctgtgagat aaggccagta gccagccccg
     4741 tcctggcagg gctgtggtga ggaggggggt gtccgtgtgg aaaactccct ttgtgagaat
     4801 ggtgcgtcct aggtgttcac caggtcgtgg ccgcctctac tccctttctc tttctccatc
     4861 cttctttcct taaagagtcc ccagtgctat ctgggacata ttcctccgcc cagagcaggg
     4921 tcccgcttcc ctaaggccct gctctgggct tctgggtttg agtccttggc aagcccagga
     4981 gaggcgctca ggcttccctg tcccccttcc tcgtccacca tctcatgccc ctggctctcc
     5041 tgccccttcc ctacaggggt tcctggctct gctctaaggg cgaattcgcg gccgctaaat
     5101 tcaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg
     5161 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5221 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctat
     5281 acgtacggca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     5341 atgtacagag tgatattatt gacacgccgg ggcgacggat ggtgatcccc ctggccagtg
     5401 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     5461 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     5521 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     5581 ggggaatata aatgtcaggc atgagattat caaaaaggat cttcacctag atccttttca
     5641 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     5701 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     5761 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     5821 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     5881 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca
     5941 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     6001 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     6061 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     6121 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     6181 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     6241 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     6301 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     6361 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     6421 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     6481 gcgaggatct cgtcgtgacc catgcatggc gatgcctgct tgccgaatat catggtggaa
     6541 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     6601 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     6661 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     6721 cttgacgagt tcttctgaat tattaacgct tacaatttcc tgatgcggta ttttctcctt
     6781 acgcatctgt gcggtatttc acaccgcatc aggtggcact tttcggggaa atgtgcgcgg
     6841 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagattatc
     6901 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6961 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7021 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7081 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7141 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7201 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7261 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7321 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7381 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7441 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7501 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7561 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7621 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7681 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7741 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7801 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7861 tcaatattat tgaagcattt atcagggtta ttgtctcatg accaaaatcc cttaacgtga
     7921 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7981 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8041 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8101 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8161 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8221 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8281 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8341 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8401 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8461 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8521 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8581 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8641 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8701 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8761 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     8821 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8881 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     8941 tcacacagga aacagctatg accatgatta cgccaagctc agaattaacc ctcactaaag
     9001 ggactagtcc tgcaggttta aacgaattcg ccctttg
//
