LOCUS       sequence-149905-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c-PK6
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..81
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             85..126
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             127..168
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             175..216
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             220..261
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             262..303
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      336..523
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(391..415)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        582..925
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aacgctagcg gtaagcctat tcctaaccct
       61 cttttgggtc tcgattctac aggtggaaaa ccaatcccca acccactcct cggccttgac
      121 tcaactggca aacccatacc aaatccttta cttggattag atagtacctc tagcggtaag
      181 cctattccta accctctttt gggtctcgat tctacaggtg gaaaaccaat ccccaaccca
      241 ctcctcggcc ttgactcaac tggcaaaccc ataccaaatc ctttacttgg attagatagt
      301 acctctagag attgaggcgc gccacttcta aataagcgaa tttcttatga tttatgattt
      361 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
      421 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
      481 aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc gctaggagat
      541 ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata
      601 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtata
      661 tttagcccac acatccccat gtataatcat ttgcatccat acattttgat ggctgcacgg
      721 cgcgaagcaa aaattacggc tcctcgctgc agacctgcga gcagggaaac gctcccctca
      781 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt
      841 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc
      901 acatcacatc cgaacataaa caaccatggg taccactctt gacgacacgg cttaccggta
      961 ccgcaccagt gtcccggggg acgccgaggc catcgaggca ctggatgggt ccttcaccac
     1021 cgaca
//
