LOCUS       sequence-149906-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c-PK6
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      569..766
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(826..843)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcttgacga cacggcttac cggtaccgca ccagtgtccc gggggacgcc gaggccatcg
       61 aggcactgga tgggtccttc accaccgaca ccgtcttccg cgtcaccgcc accggggacg
      121 gcttcaccct gcgggaggtg ccggtggacc cgcccctgac caaggtgttc cccgacgacg
      181 aatcggacga cgaatcggac gacggggagg acggcgaccc ggactcccgg acgttcgtcg
      241 cgtacgggga cgacggcgac ctggcgggct tcgtggtcgt ctcgtactcc ggctggaacc
      301 gccggctgac cgtcgaggac atcgaggtcg ccccggagca ccgggggcac ggggtcgggc
      361 gcgcgttgat ggggctcgcg acggagttcg cccgcgagcg gggcgccggg cacctctggc
      421 tggaggtcac caacgtcaac gcaccggcga tccacgcgta ccggcggatg gggttcaccc
      481 tctgcggcct ggacaccgcc ctgtacgacg gcaccgcctc ggacggcgag caggcgctct
      541 acatgagcat gccctgcccc taatcagtac tgacaataaa aagattcttg ttttcaagaa
      601 cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt
      661 gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa
      721 agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata
      781 ctaacgccgc catccagtgt taaacgagct cgaattctag gcttcctcgc tcactgactc
      841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      901 gttatccaca gaatcagggg
//
