LOCUS       sequence-149908-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c-PK3
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..85
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             89..130
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             131..172
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      205..392
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(260..284)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        451..794
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gtacgctgca ggtcgacgga tccccgggtt aattaacgct agcggtaagc ctattcctaa
       61 ccctcttttg ggtctcgatt ctacaggtgg aaaaccaatc cccaacccac tcctcggcct
      121 tgactcaact ggcaaaccca taccaaatcc tttacttgga ttagatagta cctctagaga
      181 ttgaggcgcg ccacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa
      241 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga
      301 aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagtat
      361 gaggtcgctc ttattgacca cacctctacc ggcagatccg ctaggagatc tgtttagctt
      421 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
      481 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtatat ttagcccaca
      541 catccccatg tataatcatt tgcatccata cattttgatg gctgcacggc gcgaagcaaa
      601 aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg
      661 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
      721 ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc
      781 gaacataaac aaccatgggt accactcttg acgacacggc ttaccggtac cgcaccagtg
      841 tcccggggga cgccgaggcc atcgaggcac tggatgggtc cttcaccacc gacaccgtct
      901 tccgcgtcac cgccaccggg gacggcttca ccctgcggga ggtgccggtg gacccgcccc
      961 tgaccaaggt gttccccgac gacgaatcgg acgacgaatc ggacgacggg gaggacggcg
     1021 acccggactc ccggacgttc gtc
//
