LOCUS       sequence-149927-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress MKRN1 S109D in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Lee MS, Jeong MH, Lee HW, Han HJ, Ko A, Hewitt SM, Kim JH, Chun KH, 
            Chung JY, Lee C, Cho H, Song J
  TITLE     PI3K/AKT activation induces PTEN ubiquitination and destabilization 
            accelerating tumourigenesis.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7769. doi: 10.1038/ncomms8769.
  PUBMED    26183061
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7769. doi"; pages: " 
            10.1038/ncomms8769"
COMMENT     Sequence Label: pcDNA3-FLAG MKRN1 S109D
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttgccgcc accatggatt acaaggatga cgacgataag
      121 agcgcgggcg gatccgaatt catggcggag gctgcaactc ccggaacaac agccacaaca
      181 tcaggagcag gagcggcagc ggcgacggcg gcagcagcct cccccacccc gatccccaca
      241 gtcaccgccc cgtccctggg ggcgggcgga gggggcggcg gcagcgacgg cagcggcggc
      301 ggctggacta aacaggtcac ctgcaggtat tttatgcatg gggtttgtaa ggaaggagac
      361 aactgtcgct actcgcatga cctctctgac agtccgtata gtgtagtgtg caagtatttt
      421 cagcgagggt actgtattta tggagaccgc tgcagatatg aacatgacaa accattgaaa
      481 caggaagaag caactgctac agagctaact acaaagtcat cccttgctgc ttcctcaagt
      541 ctctcatcga tagttggacc acttgttgaa atgaatacag gcgaagctga gtcaagaaat
      601 tcaaactttg caactgtagg agcaggttca gaggactggg tgaatgctat tgagtttgtt
      661 cctgggcaac cctactgtgg ccgtactgcg ccttcctgca ctgaagcacc cctgcagggc
      721 tcagtgacca aggaagaatc agagaaagag caaaccgccg tggagacaaa gaagcagctg
      781 tgcccctatg ctgcagtggg agagtgccga tacggggaga actgtgtgta tctccacgga
      841 gattcttgtg acatgtgtgg gctgcaggtc ctgcatccaa tggatgctgc ccagagatcg
      901 cagcatatc
//
