LOCUS       sequence-149932-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress MKRN1 S109A  in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Lee MS, Jeong MH, Lee HW, Han HJ, Ko A, Hewitt SM, Kim JH, Chun KH, 
            Chung JY, Lee C, Cho H, Song J
  TITLE     PI3K/AKT activation induces PTEN ubiquitination and destabilization 
            accelerating tumourigenesis.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7769. doi: 10.1038/ncomms8769.
  PUBMED    26183061
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7769. doi"; pages: " 
            10.1038/ncomms8769"
COMMENT     Sequence Label: pcDNA3-FLAG MKRN1 S109A
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             80..103
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc gccaccatgg attacaagga tgacgacgat aagagcgcgg gcggatccga
      121 attcatggcg gaggctgcaa ctcccggaac aacagccaca acatcaggag caggagcggc
      181 agcggcgacg gcggcagcag cctcccccac cccgatcccc acagtcaccg ccccgtccct
      241 gggggctggc ggagggggcg gcggcagcga cggcagcggc ggcggctgga ctaaacaggt
      301 cacctgcagg tattttatgc atggggtttg taaggaagga gacaactgtc gctactcgca
      361 tgacctctct gacagtccgt atagtgtagt gtgcaagtat tttcagcgag ggtactgtat
      421 ttatggagac cgctgcagat atgaacatgc caaaccattg aaacaggaag aagcaactgc
      481 tacagagcta actacaaagt catcccttgc tgcttcctca agtctctcat cgatagttgg
      541 accacttgtt gaaatgaata caggcgaagc tgagtcaaga aattcaaact ttgcaactgt
      601 aggagcaggt tcagaggact gggtgaatgc tattgagttt gttcctgggc aaccctactg
      661 tggccgtact gcgccttcct gcactgaagc acccctgcag ggctcagtga ccaaggaaga
      721 atcagagaaa gagcaaaccg ccgtggagac aaagaagcag ctgtgcccct atgctgcagt
      781 gggagagtgc cgatacgggg agaactgtgt gtatctccac ggagattctt gtgacatgtg
      841 tgggctgcag gtcctgcatc caatggatgc tgcccagaga tcgcagcata tcaaatcgtg
      901 cattgaggcc catgagaagg acatgg
//
