LOCUS       sequence-149935-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress MKRN1_H307E in Mammalian Cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Lee EW, Lee MS, Camus S, Ghim J, Yang MR, Oh W, Ha NC, Lane DP, Song
            J
  TITLE     Differential regulation of p53 and p21 by MKRN1 E3 ligase controls 
            cell cycle arrest and apoptosis.
  JOURNAL   EMBO J. 2009 Jul 22;28(14):2100-13. doi: 10.1038/emboj.2009.164. 
            Epub 2009 Jun 18.
  PUBMED    19536131
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2009.164"; journalName: "EMBO J"; date: "2009-07-22- 
            22"; volume: "28"; issue: "14"; pages: "2100-13"
COMMENT     Sequence Label: pcDNA3-FLAG-MKRN1_H307E
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      70..79
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             79..102
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttgccg ccaccatgga ttacaaggat gacgacgata agagcgcggg cggatccgaa
      121 ttcatggcgg aggctgcaac tcccggaaca acagccacaa catcaggagc aggagcggca
      181 gcggcgacgg cggcagcagc ctcccccacc ccgatcccca cagtcaccgc cccgtccctg
      241 ggggcgggcg gagggggcgg cggcagcgac ggcagcggcg gcggctggac taaacaggtc
      301 acctgcaggt attttatgca tggggtttgt aaggaaggag acaactgtcg ctactcgcat
      361 gacctctctg acagtccgta tagtgtagtg tgcaagtatt ttcagcgagg gtactgtatt
      421 tatggagacc gctgcagata tgaacatagc aaaccattga aacaggaaga agcaactgct
      481 acagagctaa ctacaaagtc atcccttgct gcttcctcaa gtctctcatc gatagttgga
      541 ccacttgttg aaatgaatac aggcgaagct gagtcaagaa attcaaactt tgcaactgta
      601 ggagcaggtt cagaggactg ggtgaatgct attgagtttg ttcctgggca accctactgt
      661 ggccgtactg cgccttcctg cactgaagca cccctgcagg gctcagtgac caaggaagaa
      721 tcagagaaag agcaaaccgc cgtggagaca aagaagcagc tgtgccccta tgctgcagtg
      781 ggagagtgcc gatacgggga gaactgtgtg tatctccacg gagattcttg tgacatgtgt
      841 gggctgca
//
