LOCUS       sequence-149941-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress MKRN1(110-482) in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Lee EW, Lee MS, Camus S, Ghim J, Yang MR, Oh W, Ha NC, Lane DP, Song
            J
  TITLE     Differential regulation of p53 and p21 by MKRN1 E3 ligase controls 
            cell cycle arrest and apoptosis.
  JOURNAL   EMBO J. 2009 Jul 22;28(14):2100-13. doi: 10.1038/emboj.2009.164. 
            Epub 2009 Jun 18.
  PUBMED    19536131
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2009.164"; journalName: "EMBO J"; date: "2009-07-22- 
            22"; volume: "28"; issue: "14"; pages: "2100-13"
COMMENT     Sequence Label: pcDNA3-FLAG/MKRN1(110-482)
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             80..103
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc gccaccatgg attacaagga tgacgacgat aagagcgcgg gcggatccga
      121 attcatgaaa ccattgaaac aggaagaagc aactgctaca gagctaacta caaagtcatc
      181 ccttgctgct tcctcaagtc tctcatcgat agttggacca cttgttgaaa tgaatacagg
      241 cgaagctgag tcaagaaatt caaactttgc aactgtagga gcaggttcag aggactgggt
      301 gaatgctatt gagtttgttc ctgggcaacc ctactgtggc cgtactgcgc cttcctgcac
      361 tgaagcaccc ctgcagggct cagtgaccaa ggaagaatca gagaaagagc aaaccgccgt
      421 ggagacaaag aagcagctgt gcccctatgc tgcagtggga gagtgccgat acggggagaa
      481 ctgtgtgtat ctccacggag attcttgtga catgtgtggg ctgcaggtcc tgcatccaat
      541 ggatgctgcc cagagatcgc agcatatcaa atcgtgcatt gaggcccatg agaaggacat
      601 ggagctctca tttgccgtgc agcgcagcaa ggacatggtg tgtgggatct gcatggaggt
      661 ggtctatgag aaagccaacc ccagtgagcg ccgcttcggg atcctctcca actgcaacca
      721 cacctactgt ctcaagtgca ttcgcaagtg gaggagtgct aagcaatttg agagcaagat
      781 cataaagtcc tgcccagaat gccggatcac atctaacttt gtcattccaa gtgagtactg
      841 ggtggaggag aaagaagaga agcagaaact cattctgaaa tac
//
