LOCUS       sequence-149944-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress MKRN1(1-369) in Mammalian Cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Lee EW, Lee MS, Camus S, Ghim J, Yang MR, Oh W, Ha NC, Lane DP, Song
            J
  TITLE     Differential regulation of p53 and p21 by MKRN1 E3 ligase controls 
            cell cycle arrest and apoptosis.
  JOURNAL   EMBO J. 2009 Jul 22;28(14):2100-13. doi: 10.1038/emboj.2009.164. 
            Epub 2009 Jun 18.
  PUBMED    19536131
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2009.164"; journalName: "EMBO J"; date: "2009-07-22- 
            22"; volume: "28"; issue: "14"; pages: "2100-13"
COMMENT     Sequence Label: pcDNA3-FLAG/MKRN1(1-369)
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttgccgc caccatggat tacaaggatg acgacgataa
      121 gagcgcgggc ggatccgaat tcatggcgga ggctgcaact cccggaacaa cagccacaac
      181 atcaggagca ggagcggcag cggcgacggc ggcagcagcc tcccccaccc cgatccccac
      241 agtcaccgcc ccgtccctgg gggcgggcgg agggggcggc ggcagcgacg gcagcggcgg
      301 cggctggact aaacaggtca cctgcaggta ttttatgcat ggggtttgta aggaaggaga
      361 caactgtcgc tactcgcatg acctctctga cagtccgtat agtgtagtgt gcaagtattt
      421 tcagcgaggg tactgtattt atggagaccg ctgcagatat gaacatagca aaccattgaa
      481 acaggaagaa gcaactgcta cagagctaac tacaaagtca tcccttgctg cttcctcaag
      541 tctctcatcg atagttggac cacttgttga aatgaataca ggcgaagctg agtcaagaaa
      601 ttcaaacttt gcaactgtag gagcaggttc agaggactgg gtgaatgcta ttgagtttgt
      661 tcctgggcaa ccctactgtg gccgtactgc gccttcctgc actgaagcac ccctgcaggg
      721 ctcagtgacc aaggaagaat cagagaaaga gcaaaccgcc gtggagacaa agaagcagct
      781 gtgcccctat gctgcagtgg gagagtgccg atacggggag aactgtgtgt atctccacgg
      841 a
//
