LOCUS       sequence-149947-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress MKRN1(1-280) in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Lee EW, Lee MS, Camus S, Ghim J, Yang MR, Oh W, Ha NC, Lane DP, Song
            J
  TITLE     Differential regulation of p53 and p21 by MKRN1 E3 ligase controls 
            cell cycle arrest and apoptosis.
  JOURNAL   EMBO J. 2009 Jul 22;28(14):2100-13. doi: 10.1038/emboj.2009.164. 
            Epub 2009 Jun 18.
  PUBMED    19536131
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2009.164"; journalName: "EMBO J"; date: "2009-07-22- 
            22"; volume: "28"; issue: "14"; pages: "2100-13"
COMMENT     Sequence Label: pcDNA3-FLAG/MKRN1(1-280)
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             80..103
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc gccaccatgg attacaagga tgacgacgat aagagcgcgg gcggatccga
      121 attcatggcg gaggctgcaa ctcccggaac aacagccaca acatcaggag caggagcggc
      181 agcggcgacg gcggcagcag cctcccccac cccgatcccc acagtcaccg ccccgtccct
      241 gggggcgggc ggagggggcg gcggcagcga cggcagcggc ggcggctgga ctaaacaggt
      301 cacctgcagg tattttatgc atggggtttg taaggaagga gacaactgtc gctactcgca
      361 tgacctctct gacagtccgt atagtgtagt gtgcaagtat tttcagcgag ggtactgtat
      421 ttatggagac cgctgcagat atgaacatag caaaccattg aaacaggaag aagcaactgc
      481 tacagagcta actacaaagt catcccttgc tgcttcctca agtctctcat cgatagttgg
      541 accacttgtt gaaatgaata caggcgaagc tgagtcaaga aattcaaact ttgcaactgt
      601 aggagcaggt tcagaggact gggtgaatgc tattgagttt gttcctgggc aaccctactg
      661 tggccgtact gcgccttcct gcactgaagc acccctgcag ggctcagtga ccaaggaaga
      721 atcagagaaa gagcaaaccg ccgtggagac aaagaagcag ctgtgcccct atgctgcagt
      781 gggagagtgc cgatacgggg agaactgtgt gtatctccac ggagattctt gtgacatgtg
      841 tgggctgca
//
