LOCUS       sequence-149950-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress MKRN1 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Lee EW, Lee MS, Camus S, Ghim J, Yang MR, Oh W, Ha NC, Lane DP, Song
            J
  TITLE     Differential regulation of p53 and p21 by MKRN1 E3 ligase controls 
            cell cycle arrest and apoptosis.
  JOURNAL   EMBO J. 2009 Jul 22;28(14):2100-13. doi: 10.1038/emboj.2009.164. 
            Epub 2009 Jun 18.
  PUBMED    19536131
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2009.164"; journalName: "EMBO J"; date: "2009-07-22- 
            22"; volume: "28"; issue: "14"; pages: "2100-13"
COMMENT     Sequence Label: pcDNA3-HA-MKRN1
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..109
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accatggcct cctaccctta tgatgtgcca gattatgcct ctcccgaatt
      121 catggcggag gctgcaactc ccggaacaac agccacaaca tcaggagcag gagcggcagc
      181 ggcgacggcg gcagcagcct cccccacccc gatccccaca gtcaccgccc cgtccctggg
      241 ggcgggcgga gggggcggcg gcagcgacgg cagcggcggc ggctggacta aacaggtcac
      301 ctgcaggtat tttatgcatg gggtttgtaa ggaaggagac aactgtcgct actcgcatga
      361 cctctctgac agtccgtata gtgtagtgtg caagtatttt cagcgagggt actgtattta
      421 tggagaccgc tgcagatatg aacatagcaa accattgaaa caggaagaag caactgctac
      481 agagctaact acaaagtcat cccttgctgc ttcctcaagt ctctcatcga tagttggacc
      541 acttgttgaa atgaatacag gcgaagctga gtcaagaaat tcaaactttg caactgtagg
      601 agcaggttca gaggactggg tgaatgctat tgagtttgtt cctgggcaac cctactgtgg
      661 ccgtactgcg ccttcctgca ctgaagcacc cctgcagggc tcagtgacca aggaagaatc
      721 agagaaagag c
//
