LOCUS       sequence-149956-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress MKRN1 H307E in Mammalian Cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Lee EW, Lee MS, Camus S, Ghim J, Yang MR, Oh W, Ha NC, Lane DP, Song
            J
  TITLE     Differential regulation of p53 and p21 by MKRN1 E3 ligase controls 
            cell cycle arrest and apoptosis.
  JOURNAL   EMBO J. 2009 Jul 22;28(14):2100-13. doi: 10.1038/emboj.2009.164. 
            Epub 2009 Jun 18.
  PUBMED    19536131
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2009.164"; journalName: "EMBO J"; date: "2009-07-22- 
            22"; volume: "28"; issue: "14"; pages: "2100-13"
COMMENT     Sequence Label: pcDNA3-HA-MKRN1 H307E
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc atggcctcct acccttatga tgtgccagat
      121 tatgcctctc ccgaattcat ggcggaggct gcaactcccg gaacaacagc cacaacatca
      181 ggagcaggag cggcagcggc gacggcggca gcagcctccc ccaccccgat ccccacagtc
      241 accgccccgt ccctgggggc gggcggaggg ggcggcggca gcgacggcag cggcggcggc
      301 tggactaaac aggtcacctg caggtatttt atgcatgggg tttgtaagga aggagacaac
      361 tgtcgctact cgcatgacct ctctgacagt ccgtatagtg tagtgtgcaa gtattttcag
      421 cgagggtact gtatttatgg agaccgctgc agatatgaac atagcaaacc attgaaacag
      481 gaagaagcaa ctgctacaga gctaactaca aagtcatccc ttgctgcttc ctcaagtctc
      541 tcatcgatag ttggaccact tgttgaaatg aatacaggcg aagctgagtc aagaaattca
      601 aactttgcaa ctgtaggagc aggttcagag gactgggtga atgctattga gtttgttcct
      661 gggcaaccct actgtggccg tactgcgcct tcctgcactg aagcacccct gcagggctca
      721 gtgaccaagg aagaatcaga gaaagagcaa accgccgtgg agacaaagaa gcagctgtgc
      781 ccctatgctg cagtgggaga gtgccgatac ggggagaact gtgtgtatct ccacggagat
      841 tcttgtgaca tgtgtgggct gcaggtcctg catccaatgg atgctgccca gagatcgcag
      901 catatcaaat cgtgcattga ggcccatgag aaggacatgg agctctcatt tgccgtgcag
      961 cgcagcaagg acatggtgtg tgggatctgc atgg
//
