LOCUS       sequence-149961-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c-PK3
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..565
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="TLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFTLR
                     EVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRRLT
                     VEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTLCG
                     LDTALYDGTASDGEQALYMSMPCP"
     terminator      571..768
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(828..845)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cactcttgac gacacggctt accggtaccg caccagtgtc ccgggggacg ccgaggccat
       61 cgaggcactg gatgggtcct tcaccaccga caccgtcttc cgcgtcaccg ccaccgggga
      121 cggcttcacc ctgcgggagg tgccggtgga cccgcccctg accaaggtgt tccccgacga
      181 cgaatcggac gacgaatcgg acgacgggga ggacggcgac ccggactccc ggacgttcgt
      241 cgcgtacggg gacgacggcg acctggcggg cttcgtggtc gtctcgtact ccggctggaa
      301 ccgccggctg accgtcgagg acatcgaggt cgccccggag caccgggggc acggggtcgg
      361 gcgcgcgttg atggggctcg cgacggagtt cgcccgcgag cggggcgccg ggcacctctg
      421 gctggaggtc accaacgtca acgcaccggc gatccacgcg taccggcgga tggggttcac
      481 cctctgcggc ctggacaccg ccctgtacga cggcaccgcc tcggacggcg agcaggcgct
      541 ctacatgagc atgccctgcc cctaatcagt actgacaata aaaagattct tgttttcaag
      601 aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc
      661 gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag
      721 aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga
      781 tactaacgcc gccatccagt gttaaacgag ctcgaattct aggcttcctc gctcactgac
      841 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      901 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      961 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct c
//
