LOCUS       sequence-149962-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress MKRN1 110-482 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Lee EW, Lee MS, Camus S, Ghim J, Yang MR, Oh W, Ha NC, Lane DP, Song
            J
  TITLE     Differential regulation of p53 and p21 by MKRN1 E3 ligase controls 
            cell cycle arrest and apoptosis.
  JOURNAL   EMBO J. 2009 Jul 22;28(14):2100-13. doi: 10.1038/emboj.2009.164. 
            Epub 2009 Jun 18.
  PUBMED    19536131
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2009.164"; journalName: "EMBO J"; date: "2009-07-22- 
            22"; volume: "28"; issue: "14"; pages: "2100-13"
COMMENT     Sequence Label: pcDNA3-HA-MKRN1 110-482
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..109
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accatggcct cctaccctta tgatgtgcca gattatgcct ctcccgaatt
      121 catgaaacca ttgaaacagg aagaagcaac tgctacagag ctaactacaa agtcatccct
      181 tgctgcttcc tcaagtctct catcgatagt tggaccactt gttgaaatga atacaggcga
      241 agctgagtca agaaattcaa actttgcaac tgtaggagca ggttcagagg actgggtgaa
      301 tgctattgag tttgttcctg ggcaacccta ctgtggccgt actgcgcctt cctgcactga
      361 agcacccctg cagggctcag tgaccaagga agaatcagag aaagagcaaa ccgccgtgga
      421 gacaaagaag cagctgtgcc cctatgctgc agtgggagag tgccgatacg gggagaactg
      481 tgtgtatctc cacggagatt cttgtgacat gtgtgggctg caggtcctgc atccaatgga
      541 tgctgcccag agatcgcagc atatcaaatc gtgcattgag gcccatgaga aggacatgga
      601 gctctcattt gccgtgcagc gcagcaagga catggtgtgt gggatctgca tggaggtggt
      661 ctatgagaaa gccaacccca gtgagcgccg cttcgggatc ctctccaact gcaaccacac
      721 ctactgtctc aagtgcattc gcaagtggag gagtgctaag caatttgaga gcaagatcat
      781 aaagtcctgc ccagaatgcc ggatcacatc taactttgtc attccaagtg agtactgggt
      841 ggaggagaaa gaagagaagc agaaactcat tctgaaatac aaggaggcaa tgagcaacaa
      901 ggcgtgcagg tattttgatg aa
//
