LOCUS       sequence-149967-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c-FL3
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..68
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             72..119
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     terminator      152..339
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(207..231)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        414..757
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 ctgcaggtcg acggatcccc gggttaatta acgctagcat gggtgactac aaggacgacg
       61 atgacaaggg agattacaaa gatgacgacg ataaggacta caaggacgac gatgacaagt
      121 ctagagattg aggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat
      181 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
      241 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
      301 atagtatgag gtcgctctta ttgaccacac ctctaccggc agatccgcta gggataacag
      361 ggtaatatag atctgtttag cttgcctcgt ccccgccggg tcacccggcc agcgacatgg
      421 aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact
      481 gtcgcccgta tatttagccc acacatcccc atgtataatc atttgcatcc atacattttg
      541 atggctgcac ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc gagcagggaa
      601 acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa
      661 aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa atcttgctag
      721 gatacagttc tcacatcaca tccgaacata aacaaccatg ggtaccactc ttgacgacac
      781 ggcttaccgg taccgcacca gtgtcccggg ggacgccgag gccatcgagg cactggatgg
      841 gtccttcacc accgacaccg tcttccgcgt caccgccacc ggggacggct tcaccctgcg
      901 ggaggtgccg gtggacccgc ccctgaccaa ggtgttcccc gacgacgaat cggacgacga
      961 atcggacgac ggggaggacg gcgacccgga ctcccggacg ttcgtcgcgt acggggacga
     1021 cggcgacctg g
//
