LOCUS       sequence-149973-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To purify GST-tagged MKRN1 recombinant protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Lee EW, Lee MS, Camus S, Ghim J, Yang MR, Oh W, Ha NC, Lane DP, Song
            J
  TITLE     Differential regulation of p53 and p21 by MKRN1 E3 ligase controls 
            cell cycle arrest and apoptosis.
  JOURNAL   EMBO J. 2009 Jul 22;28(14):2100-13. doi: 10.1038/emboj.2009.164. 
            Epub 2009 Jun 18.
  PUBMED    19536131
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2009.164"; journalName: "EMBO J"; date: "2009-07-22- 
            22"; volume: "28"; issue: "14"; pages: "2100-13"
COMMENT     Sequence Label: pGEX 4T-1/MKRN1
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctggttccgc gtggatcccc ggaattcatg gcggaggctg caactcccgg aacaacagcc
       61 acaacatcag gagcaggagc ggcagcggcg acggcggcag cagcctcccc caccccgatc
      121 cccacagtca ccgccccgtc cctgggggcg ggcggagggg gcggcggcag cgacggcagc
      181 ggcggcggct ggactaaaca ggtcacctgc aggtatttta tgcatggggt ttgtaaggaa
      241 ggagacaact gtcgctactc gcatgacctc tctgacagtc cgtatagtgt agtgtgcaag
      301 tattttcagc gagggtactg tatttatgga gaccgctgca gatatgaaca tagcaaacca
      361 ttgaaacagg aagaagcaac tgctacagag ctaactacaa agtcatccct tgctgcttcc
      421 tcaagtctct catcgatagt tggaccactt gttgaaatga atacaggcga agctgagtca
      481 agaaattcaa actttgcaac tgtaggagca ggttcagagg actgggtgaa tgctattgag
      541 tttgttcctg ggcaacccta ctgtggccgt actgcgcctt cctgcactga agcacccctg
      601 cagggctcag tgaccaagga agaatcagag aaagagcaaa ccgccgtgga gacaaagaag
      661 cagctgtgcc cctatgctgc agtgggagag tgccgatacg gggagaactg tgtgtatctc
      721 cacggagatt cttgtgacat gtgtgggctg caggtcctgc atccaatgga tgctgcccag
      781 agatcgcagc atatcaaatc gtgcattgag gcccatgaga aggacatgga gctctcattt
      841 gccgtgcagc gcagca
//
