LOCUS       sequence-149977-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress p21 90-164 in Mammalian Cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim 
            KP, Song J
  TITLE     Stabilization of p21 (Cip1/WAF1) following Tip60-dependent 
            acetylation is required for p21-mediated DNA damage response.
  JOURNAL   Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. 
            Epub 2012 Dec 14.
  PUBMED    23238566
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2012.159"; journalName: "Cell Death Differ"; date: 
            "2013-04"; volume: "20"; issue: "4"; pages: "620-9"
COMMENT     Sequence Label: pCS3-MT-BX-6MYC p21 90-164
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(324..353)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(387..416)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(426..455)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(465..494)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(504..533)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(543..572)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(622..640)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(623..640)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atacgactca ctatagttct agatccggtg gatcccgggc ccgcggtacc gtcgagttag
       61 ggcttcctct tggagaagat cagccggcgt ttggagtggt agaaatctgt catgctggtc
      121 tgccgccgtt ttcgaccctg agagtctcca ggtccacctg gggacccttc agcctgctcc
      181 cctgagcgag gcacaagggt acaagacagt gacaggtcca catggtcttc ctctgctgtc
      241 ccctgcagca gagcaggtga ggtgccaggc cgcctgcctc ctcccaacat gaattcgaag
      301 cttgagctcg agatctctaa attcaagtcc tcttcagaaa tgagcttttg ctccatggtg
      361 aggtcgccca agctctccat ttcattcaag tcctcttcag aaatgagctt ttgctccatt
      421 tcattcaagt cctcttcaga aatgagcttt tgctccattt cattcaagtc ctcttcagaa
      481 atgagctttt gctccatttc attcaagtcc tcttcagaaa tgagcttttg ctccatttca
      541 ttcaagtcct cttcagaaat gagcttttgc tccatagctt taaatcgatg ggatcctgca
      601 aaaagaacaa gtagcttgta ttctatagtg tcacctaaat caagcttgct ccgacgtccc
      661 caggcagaat ggcggttccc taaacgagca ttgcttatat agacctccca ttaggcacgc
      721 ctaccgccca tttacgtcaa tggaacgccc atttgcgtca ttgcccctcc ccattgacgt
      781 caatggggat gtacttggca gccatcgcgg ggccatttac cgccattgac gtcaatggga
//
