LOCUS       sequence-149979-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To detect localization of MKRN1 in live mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Ko A, Shin JY, Seo J, Lee KD, Lee EW, Lee MS, Lee HW, Choi IJ, Jeong
            JS, Chun KH, Song J
  TITLE     Acceleration of gastric tumorigenesis through MKRN1-mediated 
            posttranslational regulation of p14ARF.
  JOURNAL   J Natl Cancer Inst. 2012 Nov 7;104(21):1660-72. doi: 
            10.1093/jnci/djs424. Epub 2012 Oct 25.
  PUBMED    23104211
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jnci/djs424"; journalName: "J Natl Cancer Inst"; date: 
            "2012-11-7- 7"; volume: "104"; issue: "21"; pages: "1660-72"
COMMENT     Sequence Label: pEGFP-MKRN1 WT
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(67..270)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(96..116)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        271..574
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(829..848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtagcgc tagcggatct gacggttcac
       61 taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      121 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
      181 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
      241 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
      301 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      361 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
      421 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
      481 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
      541 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
      601 taatgacccc gtaattgatt actattaata actaatgcat ggcggtaata cggttatcca
      661 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      721 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      901 acctgtccgc c
//
