LOCUS       sequence-149983-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress RIPK1 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-FLAG-RIPK1
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             80..103
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc gccaccatgg attacaagga tgacgacgat aagagcgcgg gcggatccga
      121 attcatgcaa ccagacatgt ccttgaatgt cattaagatg aaatccagtg acttcctgga
      181 gagtgcagaa ctggacagcg gaggctttgg gaaggtgtct ctgtgtttcc acagaaccca
      241 gggactcatg atcatgaaaa cagtgtacaa ggggcccaac tgcattgagc acaacgaggc
      301 cctcttggag gaggcgaaga tgatgaacag actgagacac agccgggtgg tgaagctcct
      361 gggcgtcatc atagaggaag ggaagtactc cctggtgatg gagtacatgg agaagggcaa
      421 cctgatgcac gtgctgaaag ccgagatgag tactccgctt tctgtaaaag gaaggataat
      481 tttggaaatc attgaaggaa tgtgctactt acatggaaaa ggcgtgatac acaaggacct
      541 gaagcctgaa aatatccttg ttgataatga cttccacatt aagatcgcag acctcggcct
      601 tgcctccttt aagatgtgga gcaaactgaa taatgaagag cacaatgagc tgagggaagt
      661 ggacggcacc gctaagaaga atggcggcac cctctactac atggcgcccg agcacctgaa
      721 tgacgtcaac gcaaagccca cagagaagtc ggatgtgtac agctttgctg tagtactctg
      781 ggcgatattt gcaaataagg agccatatga aaa
//
