LOCUS       sequence-149991-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear fluorescent reporter in mammalian cells, phiC31 attB for 
            integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can 
            be KRAB or DNMT3B)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Bintu L, Yong J, Antebi YE, McCue K, Kazuki Y, Uno N, Oshimura M, 
            Elowitz MB
  TITLE     Dynamics of epigenetic regulation at the single-cell level.
  JOURNAL   Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
  PUBMED    26912859
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-02-12- 12"; volume: "351"; 
            issue: "6274"; pages: "720-4"
COMMENT     Sequence Label: PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     276..295
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        276..294
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(346..597)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(388..409)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     737..756
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcat gaacagactg
      121 tgaggactga ggggcctgaa atgagccttg ggactgtgaa tctaaaatac acaaacaatt
      181 agaatcagta gtttaacaca ttatacactt aaaaattgga tctccattcg ccattcaggc
      241 tgcgcaactg ttgggaaggg cgatcgagtg aattgtaata cgactcacta tagggcgaat
      301 tgggtacact tacctggtac cgggcccccc ctcgaggtcg acctacttgt acagctcgtc
      361 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      421 ctcgttgggg tctttgctca gggcggactg gtagctcagg tagtggttgt cgggcagcag
      481 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
      541 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc
      601 catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
      661 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
      721 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
      781 gacgtagcct tcggg
//
