LOCUS       sequence-149994-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry 
            marker in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Bintu L, Yong J, Antebi YE, McCue K, Kazuki Y, Uno N, Oshimura M, 
            Elowitz MB
  TITLE     Dynamics of epigenetic regulation at the single-cell level.
  JOURNAL   Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
  PUBMED    26912859
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-02-12- 12"; volume: "351"; 
            issue: "6274"; pages: "720-4"
COMMENT     Sequence Label: pEx1-pEF-H2B-mCherry-T2A-rTetR-EED
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aaattggatc tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcgagtgaa
       61 ttgtaatacg actcactata gggcgaattg ggtacactta cctggtacct tatcgaagtc
      121 gatcccagcg ccaaatgctg gcatcatcgc agacagctat gaggatgctg ctatccctac
      181 tgaaactggt ttgtcgaata gccgcgccac atttatgatg ggtcagtgtt gtgcatttgg
      241 ctttatgagg atcttctact tctaaatccc aaacatacag tttgccaacc tgattgccca
      301 atgcaagcat cttttgccag aaatccatag aaaacctcat gtaccaaatg tcacactggc
      361 tgtaatcaaa tcgcccaaga atagtcacat tagactcact aggtttaatt ttatctatat
      421 catcctccat tttgccaggt ttccagcata caatggcatt ttcacaagac ttggaaagta
      481 tcaaatcgcc taaccatcgc acacaatcaa cataattcct atgtatgtct ctggtagaaa
      541 agtcaggaaa gtggattttc tgggaaataa atggcctgtt agttttgttt gggttataat
      601 cataagactc cttaattgca ttcatcatcc tctttgagtt gattctccac agtttaagag
      661 agtgatccat accacaggac attatttttt cacccaaaag atcataatca gcactcagaa
      721 cttcatctct gtgcccttcc acacctccga atattgccac aagagtgtct gttt
//
