LOCUS       sequence-149999-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry
            marker in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Bintu L, Yong J, Antebi YE, McCue K, Kazuki Y, Uno N, Oshimura M, 
            Elowitz MB
  TITLE     Dynamics of epigenetic regulation at the single-cell level.
  JOURNAL   Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
  PUBMED    26912859
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-02-12- 12"; volume: "351"; 
            issue: "6274"; pages: "720-4"
COMMENT     Sequence Label: pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aaattggatc tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcgagtgaa
       61 ttgtaatacg actcactata gggcgaattg ggtacactta cctggacctt acaggggcgg
      121 ctcctcttcc atgggctcct catctggtct cttttcggcg ggcttcacgc ccacggacag
      181 cgaggccatg gcggtgaccg tctcggcttc ttcgttctcg caagtctgag cctcgatcag
      241 agaacgcccc gctgtggagg ttgtgcgctg caggcagcgc cagtacttgc tgtggatctc
      301 catgactttc tccatggaac ggacagcgtt tgcattgggt ctttgctgta aaaccttttc
      361 tgggagagga tcaagctcgt ttcccagcaa ggcagaaaca catgcttccg aggcgtcgca
      421 aatggcggtc aggtcgtggc ctccctcgag ggccaggaca atccggccgc cagccaggcc
      481 catcagctgc ttcgtcaggt acccgaagca tctggcggag aggttgtagc ccccaagagg
      541 ggtggggtgg ccctccacgg catcgaagcc tgatgacacc agcaccacat ccggggcaaa
      601 ctcgctggcg atcggcatga ccaccgttct gaaggccgcc aagtactcag cgtctcccat
      661 gggggggtcc aggccgccgg tgaaagccat gttgacgttg aaacccacgc cgggccctgt
      721 gcccacctca tcaggagccc cgctgcctgg gaagaagtt
//
