LOCUS       sequence-150005-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression flag-USP19 1-1290 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: pRK-flag-USP19 1-1290
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        221..239
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     221..238
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     252..271
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      311..320
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             320..343
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
       61 accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga
      121 gtgacgtaag taccgcctat agagtctata ggcccacccc cttggcttcg ttagaacgcg
      181 gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa
      241 taacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac tgcacctcgg
      301 ttctaagctt gccgccatgg actacaagga cgacgatgac aaggggtcga ccatgtctgg
      361 cggggccagt gccacaggcc caaggagagg gcccccagga ctggaggaca ccactagtaa
      421 gaagaagcag aaggatcgag caaaccagga gagcaaggat ggagatccta ggaaagagac
      481 agggtctcga tatgttgccc aggctggtct tgaacctctg gcctcaggtg atccttctgc
      541 ctcagcctcc catgcagctg ggatcacagg ctcacgccac cgtacccggc tgttctttcc
      601 ttcatcgtca gggtcagcat ccactcctca agaggagcag accaaagagg gagcttgtga
      661 agaccctcat gatctcttgg ctactcccac tccagagttg ttgctcgatt ggaggcagag
      721 tgcagaagag gtgattgtca agcttcgtgt gggagtaggt cccctgcagc tggaggatgt
      781 agatgctgct ttcacagata cagactgtgt ggtgcggttt gcaggtggtc agcagtggg
//
