LOCUS       sequence-150009-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry 
            marker in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Bintu L, Yong J, Antebi YE, McCue K, Kazuki Y, Uno N, Oshimura M, 
            Elowitz MB
  TITLE     Dynamics of epigenetic regulation at the single-cell level.
  JOURNAL   Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
  PUBMED    26912859
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-02-12- 12"; volume: "351"; 
            issue: "6274"; pages: "720-4"
COMMENT     Sequence Label: pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..84
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        65..83
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(177..302)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
ORIGIN
        1 aaaattggat ctccattcgc cattcaggct gcgcaactgt tgggaagggc gatcgagtga
       61 attgtaatac gactcactat agggcgaatt gggtacactt acctggtacc ttaccaggga
      121 tcctctcctt gctgcaacag ggagatcacc tttggtttgg taaacaggaa tcctgccatg
      181 gaggccaggt tgctgtaatt ctccagcatc acctcacggt acaggcttct ctgagacaga
      241 tccagcttct tccactcgtc ccgagtaaag agcacagcca catcttcaaa tgtcactgac
      301 actgctaggc atgcgctacc gctgccgcta cccccgccgc tttcgcactt tagctgtttc
      361 tccaggccac atatgattag ttccaggccg aaaaggaagg caggttcggc tccctgccgg
      421 tcgaacagct caattgcttg tctcagaagt gggggcatag aatcggtggt aggtgtctct
      481 ctttcctctt ttgctacttg atgctcctgt tcctccaata cgcagcccag tgtaaagtgg
      541 cccacggcgg acagagcgta cagtgcgttc tccagggaga agccttgctg acacaggaac
      601 gcgagctgat tttccagggt ttcgtactgt ttctctgttg ggcgggtgcc gagatgcact
      661 ttagccccgt cgcgatgtga gaggagagca cagcggtatg acttggcgtt gttccgcaga
      721 aa
//
