LOCUS       sequence-150011-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses maltose binding protein (MBP) SpyCatcher fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Min D, Arbing MA, Jefferson RE, Bowie JU
  TITLE     A simple DNA handle attachment method for single molecule mechanical
            manipulation experiments.
  JOURNAL   Protein Sci. 2016 Aug;25(8):1535-44. doi: 10.1002/pro.2952. Epub 
            2016 Jun 6.
  PUBMED    27222403
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2952"; 
            journalName: "Protein Sci"; date: "2016-08"; volume: "25"; issue: 
            "8"; pages: "1535-44"
COMMENT     Sequence Label: pMBP-SpyCatcher
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(103..438)
                     /codon_start=1
                     /product="truncated version of the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyCatcher
                     /note="forms a covalent isopeptide bond with the SpyTag 
                     peptide (Zakeri et al., 2012)"
                     /translation="DTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGKELAGATMEL
                     RDSSGKTISTWISDGQVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNGK
                     ATKGDAHI"
     CDS             complement(448..468)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(490..507)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(529..552)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      660..669
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atcctcaagc atcaaccatt taaatatgag cgtcaccttt
      121 agttgctttg ccatttacag taacctgacc ttgctcatta actgtaaagg taatagcagt
      181 tgctacctca taaccgtctg gtgctgcggt ttcgacaaat gtatattttc ctggatacag
      241 gtagaaatct ttcacttgtc catctgaaat ccatgtacta atagttttac cagatgaatc
      301 acgcaactcc atagttgcac cagctaactc tttgccgtcc tcatcacgtt ttgagaattt
      361 aatatgggta gcactatctt cttcaattgt catatcaccg gactgacctt gctcacttga
      421 taaacctgat aaggtatcac cacaaccacc ctgaaaatac aggttttcgg tcgttgggat
      481 gtcgtaatcg tgatggtgat ggtgatggta gtacgacatg ccattagtct gcgcgtcttt
      541 cagggcttca tcgacagtct gacgaccgct ggcggcgttg atcaccgcag tacgcacggc
      601 ataccagaaa gcggacatct gcgggatgtt cggcatgatt tcacctttct gggcgttttc
      661 catggtggcg gcaatacgtg gatctttcgc caactcttcc tcgtaagact tcagcgctac
      721 ggcacccagc ggtttgtctt tattaaccgc ttccagacct tcatcagtca gcagatagtt
      781 ttcgaggaac tcttttg
//
