LOCUS       sequence-150013-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression flag-USP19 494-1290 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: pRK-flag-USP19 494-1290
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        227..245
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     227..244
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     258..277
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      317..326
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             326..349
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttagtgaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
       61 accgggaccg atccagcctc cgcggccggg aacggtgcat tggaacgcgg attccccgtg
      121 ccaagagtga cgtaagtacc gcctatagag tctataggcc cacccccttg gcttcgttag
      181 aacgcggcta caattaatac ataaccttat gtatcataca catacgattt aggtgacact
      241 atagaataac atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca
      301 cctcggttct aagcttgccg ccatggacta caaggacgac gatgacaagg ggtcgacccc
      361 aggcttcact ggccttgtca atttaggcaa cacctgcttc atgaacagcg tcattcagtc
      421 tctgtccaac actcgggaac tccgggactt cttccatgac cgctcctttg aggctgagat
      481 caactacaac aacccactag ggactggtgg gcgtctggcc attggctttg ccgtgctgct
      541 tcgggcgctg tggaagggca cccaccatgc cttccagcct tccaagttga aggccattgt
      601 ggcgagtaag gccagccagt tcacaggcta tgcacagcat gatgcccagg agttcatggc
      661 tttcctgctg gatgggctgc acgaggacct gaatcgcatt cagaacaagc cctacacaga
      721 gaccgtggat tcagatgggc ggcccgatga ggtggtagct gaggaagcat ggcagcggca
      781 caagatgagg aatgactctt tcatcgtgga cctatttcag gg
//
