LOCUS       sequence-150020-        4947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Monomeric near-infrared fluorescent protein miRFP703 in bacterial 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, 
            Verkhusha VV
  TITLE     Bright monomeric near-infrared fluorescent proteins as tags and 
            biosensors for multiscale imaging.
  JOURNAL   Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.
  PUBMED    27539380
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-19"; volume: "7:12405. doi"; pages: " 
            10.1038/ncomms12405"
COMMENT     Sequence Label: pBAD/His-miRFP703
FEATURES             Location/Qualifiers
     source          1..4947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             328..345
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             355..1302
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     primer_bind     complement(1348..1365)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1518..1604
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1696..1723
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1742..1833
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1834..1902,1903..2694)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2052..2071)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2865..3453
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3354..3373
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3607..3624
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3639..3779
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3705..3724)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3865..3887
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4043..4921)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat ggggagccac catcaccatc accatggcag atctatggta
      361 gcaggtcatg cctctggcag ccccgcattc gggaccgcct ctcattcgaa ttgcgaacat
      421 gaagagatcc acctcgccgg ctcgatccag ccgcatggcg cgcttctggt cgtcagcgaa
      481 catgatcatc gcgtcatcca ggccagcgcc aacgccgcgg aatttctgaa tctcggaagc
      541 gtactcggcg ttccgctcgc cgagatcgac ggcgatctgt tgatcaagat cctgccgcat
      601 ctcgatccca ccgccgaagg catgccggtc gcggtgcgct gccggatcgg caatccctct
      661 acggagtact gcggtctgat gcatcggcct ccggaaggcg ggctgatcat cgaactcgaa
      721 cgtgccggcc cgtcgatcga tctgtcaggc acgctggcgc cggcgctgga gcggatccgc
      781 acggcgggtt cactgcgcgc gctgtgcgat gacaccgtgc tgctgtttca gcagtgcacc
      841 ggctacgacc gggtgatggt gtatcgtttc gatgagcaag gccacggcct ggtattctcc
      901 gagtgccatg tgcctgggct cgaatcctat ttcggcaacc gctatccgtc gtcgctggtc
      961 ccgcagatgg cgcggcagct gtacgtgcgg cagcgcgtcc gcgtgctggt cgacgtcacc
     1021 tatcagccgg tgccgctgga gccgcggctg tcgccgctga ccgggcgcga tctcgacatg
     1081 tcgggctgct tcctgcgctc gatgtcgccg atccatctgc agttcctgaa ggacatgggc
     1141 gtgcgcgcca ccctggcggt gtcgctggtg gtcggcggca agctgtgggg cctggttgtc
     1201 tgtcaccatt atctgccgcg cttcatccgt ttcgagctgc gggcgatctg caaacggctc
     1261 gccgaaagga tcgcgacgcg gatcaccgcg cttgagagct aagaattcga agcttggctg
     1321 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
     1381 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
     1441 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt
     1501 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1561 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
     1621 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
     1681 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
     1741 ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1801 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     1861 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1921 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1981 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     2041 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     2101 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     2161 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     2221 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     2281 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     2341 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     2401 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     2461 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     2521 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     2581 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     2641 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     2701 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2761 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2821 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2881 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2941 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3001 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3061 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3121 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3181 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3241 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3301 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3361 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3421 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3481 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3541 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3601 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     3661 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     3721 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     3781 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     3841 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     3901 caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa ggcgaagcgg catgcataat
     3961 gtgcctgtca aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt
     4021 caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc actttttctt
     4081 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga
     4141 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg
     4201 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
     4261 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg
     4321 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
     4381 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca
     4441 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
     4501 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
     4561 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
     4621 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt
     4681 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc
     4741 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
     4801 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
     4861 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca
     4921 tacttttcat actcccgcca ttcagag
//
