LOCUS       sequence-150036-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Small fragment of split HRP fused to the extracellular terminus of 
            NLG1. This is a lentiviral plasmid, but can also be used for 
            transfections. Syanpsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: Synapsin sHRPb-NLG in FSW lentiviral vector
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             466..507
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cgcagtctag acgccaccat ggcacttccc agatgcatgt ggccaaatta tgtttggaga
       61 gctatgatgg catgtgtggt ccacagggga tccggtgccc cattgactct ctgcttgttg
      121 ggatgtttgc tacagacttt tcacgtactc tctcaaaaga ccggtaactt gtctgcactg
      181 gttgactttg acctgcgcac cccaaccatc ttcgacaata agtattacgt caatctggag
      241 gaacagaagg gcctgattca gtccgaccag gagctgttca gttccccaga tgctaccgac
      301 actattcctc ttgtgcgcag cttcgctaat agcacacaaa cctttttcaa tgctttcgtg
      361 gaagctatgg atcggatggg aaatataacg cccctgacgg gtacgcaggg acagataaga
      421 cggaactgta gggtcgtgaa cagcaactct ggctcgggct cgggcggcaa gcccatcccc
      481 aaccccctgc tgggcctgga cagcacccaa ttgttggatg atgtagaccc attggttact
      541 actaactttg gcaagattag gggaattaag aaagaactca ataatgaaat tttgggtcct
      601 gtcattcagt ttcttggggt tccatatgcc gctccaccaa caggagaaca tcgtttccag
      661 cctccagaac caccatctcc ctggtctgac atccggaacg ccactcagtt tgctcctgta
      721 tgtccccaga atatcattga tggcagattg cctgaagtta tgcttcctgt gtggttcact
//
