LOCUS       sequence-150040-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression flag-GFP 1-10 tetra Cys in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: pRK-flag-GFP 1-10 tetra Cys
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..80
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        100..118
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     100..117
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(156..173)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca
       61 actagaaggc acagtcgagg ctgatcagcg agctctagca tttaggtgac actatagaat
      121 agggccctct agatgcatgc tcgagcggcc gcttagcagc aaccagggca gcagccgccg
      181 ctgcctttct cgtttgggtc tttgctcagc actgtctgtg tgctcaggta gtggttgtct
      241 ggcagcagca ctgggccgtc gccgattggt gtgttctgct ggtagtggtc ggcgagctgc
      301 acgctgccgt cctccacgtt gtgtctcact gtgaagttgg ctttgatgcc gtttttctgt
      361 ttgttggctg tgatgtacac gttgtggctg ttgaagttgt actccagttt gtggcccagg
      421 atgttgccgt cctctttgaa gtctgtgcct ttcagctcga ttctgttcac cagtgtgtcg
      481 ccctcgaatt tcaccacggc tcttgttttg tatttgccgt cgtctttgaa gctgattgtt
      541 ctctcctgca cgtagccctc tggcatggcg cttttgaaga agtcgtgtct tttcatgtgg
      601 tccggatatc tgctgaagca ctgcacgccg tatgtcagtg ttgtcactag tgttggccag
      661 ggcactggca gtttgcctgt tgtgcagatg aatttcagtg tcagtttgcc gattgtggcg
      721 tcgccctcgc cctcgcctct cacgctgaat t
//
