LOCUS       sequence-150041-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression flag-GFP 1-10 tetra Cys in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: pRK-flag-GFP 1-10 tetra Cys
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        221..239
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     221..238
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     252..271
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      311..320
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             320..343
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
       61 accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga
      121 gtgacgtaag taccgcctat agagtctata ggcccacccc cttggcttcg ttagaacgcg
      181 gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa
      241 taacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac tgcacctcgg
      301 ttctaagctt gccgccatgg actacaagga cgacgatgac aaggggtcga ccatggtttc
      361 gaaaggcgag gagctgttca caggcgtggt gccaatcctg gtggagctgg acggcgacgt
      421 gaacggccac aaattcagcg tgagaggcga gggcgagggc gacgccacaa tcggcaaact
      481 gacactgaaa ttcatctgca caacaggcaa actgccagtg ccctggccaa cactagtgac
      541 aacactgaca tacggcgtgc agtgcttcag cagatatccg gaccacatga aaagacacga
      601 cttcttcaaa agcgccatgc cagagggcta cgtgcaggag agaacaatca gcttcaaaga
      661 cgacggcaaa tacaaaacaa gagccgtggt gaaattcgag ggcgacacac tggtgaacag
      721 aatcgagctg aaaggcacag acttcaaaga ggacggcaac atcctgggcc acaaactgga
      781 gtacaacttc aacagccaca acgtgtacat cacagccaac aaacagaaaa acggcatcaa
      841 agccaacttc acagtgagac aca
//
