LOCUS       sequence-150048-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Large fragment of split HRP targeted for expression in the lumen of 
            the endoplasmic reticulum (ER). CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: pDisplay sHRPa-KDEL
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     134..193
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             218..241
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattcggctt
      121 ggggatatcc accatggaga cagacacact cctgctatgg gtactgctgc tctgggttcc
      181 aggttccact ggtgacgggg cccagccggc cagatctgat tataaggacg atgatgacaa
      241 acagcttact ccaactttct acgataactc ttgtcctaac gtgagcaaca tcgtgcgaga
      301 tattatcgtg aacgagttgc ggtctgatcc gaggatagcc gcttcaattt tgcggctgca
      361 tttccacgat tgttttgtaa acggctgtga tgcttccatc ctcctcgaca ataccacctc
      421 tttcaggact gaaaaggatg ccttcggtaa cgcgaatagt gctagagggt tttccgtgat
      481 cgatagaatg aaagccgctg tggagagcgc ctgccccggg acagttagtt gtgcagacct
      541 tctgaccata gcggcccagc aatccgtgac gctggccggt ggccccagct ggagggtccc
      601 cctgggaagg agggactctc tgcaggcttt tctggacttg gcaaacgcaa acctgcctgc
      661 tcccttcttc actcttcccc agctgaagga ttccttcagg aatgtcggcc tgaaccgaag
      721 tagtgatttg gtggctctgt ccggaggcca cacattcggc aagagtcagt gcaggttcat
      781 catggacaga ctgtataact tttccaacac cggcttgcct gaccctac
//
