LOCUS       sequence-150053-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Large fragment of split HRP targeted for expression in the lumen of 
            the endoplasmic reticulum (ER). CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: pDisplay sHRPa-KDEL
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    187..411
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     400..417
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(439..585)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(589..618)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      121 tcctgcgtta tcccccgatt ctgtggataa ccgtattacc gccactggtt ctttccgcct
      181 cagaagccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc
      241 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg
      301 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg
      361 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg
      421 atctcgagcg gccgcctaac gtggcttctt ctgccaaagc atgatgagga tgataaggga
      481 gatgatggtg agcaccacca gggccaggat ggctgagatc accaccacct taaagggcaa
      541 ggagtgtggc accacgatga cctcctgcgt gtcctggccc acagcattca gatcctcttc
      601 tgagatgagt ttttgttcgt cgactggcgc gccacattat tacagctcgt ccttgccgct
      661 gccgccattg agcggacaca gtcctctgag agtctgcaag tatgttgtgt tcagggtagg
      721 gtcaggcaag ccggtgttgg aaaagttata cagtctgtcc atgatgaacc tgcactgact
      781 cttgccgaat gtgtggcctc cggacagagc caccaaatca ctacttcggt tcaggccgac
      841 attcctg
//
