LOCUS       sequence-150056-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 
            (orange) fluorophore separated by a self-cleaving 2A peptide for use
            with 5' promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: pME-H2B-mCerulean3-P2A-mKOFP2-CAAX
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(826..845)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagcaggct gaattcgccg ccaccatgcc tgagcctagc
      121 aagagcgccc ctgcccctaa gaagggaagc aagaaggcca tcaccaaggc ccagaagaag
      181 gacggaaaga agcgcaagag aagccgcaaa gagtcttaca gcatctacgt gtacaaggtg
      241 ctgaaacagg tgcaccctga caccggaatc agcagcaagg ccatgggaat catgaacagc
      301 ttcgtgaacg acatcttcga gagaatcgcc ggcgaggcca gcagactggc ccactacaac
      361 aagagaagca ccatcaccag ccgcgagatc cagaccgccg tcagactgct gctgcctggc
      421 gagctggcca agcacgccgt gtctgaggga acaaaggccg tgaccaagta cacaagcagc
      481 aagggcggag gaagcggagg cggaagcggc ggaggatcta tggtgtctaa gggcgaggaa
      541 ctgttcaccg gcgtggtgcc tatcctggtg gaactggacg gcgacgtgaa cggacacaag
      601 ttcagcgtgt ccggcgaggg cgaaggcgac gccacctacg gaaagctgac cctgaagttc
      661 atctgcacca ccggaaagct gcctgtgcct tggcctaccc tggtcaccac cctgagctgg
      721 ggagtgcagt gcttcgccag ataccctgac cacatgaagc agcacgattt cttcaagagc
      781 gccatgcctg agggatacgt gcaggaaaga accatcttct tcaaggacga cggcaactac
      841 aagaccagag ccgaagtgaa gttcgagggc gacaccctgg tcaacagaat cgagctgaag
      901 ggaatcgact tcaaagagga cggaaacatc ctgggacaca agctggagta caacgccatc
      961 cacggaaacg tgtacatcac cgccgacaag cagaagaacg gaatcaaggc caacttcggc
     1021 ctgaactgca acatcgagga cggaagc
//
