LOCUS       sequence-150057-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 
            (orange) fluorophore separated by a self-cleaving 2A peptide for use
            with 5' promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: pME-H2B-mCerulean3-P2A-mKOFP2-CAAX
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(182..835)
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             complement(842..898)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgataagc aatgctttct tataatgcca actttgtaca agaaagctgg gtactagttc
      121 agctcagcac gcatttgcag ctcatgcatc caggtccgct ctcgtctgga gggttcagct
      181 tgctgtgagc cacggcgtcc tcgacctgct cggtgatgtt tccctcggtc ttgcggacca
      241 gtctgtgtcc gatgtagtgg tcgcctggca tttccaggat ttctttggcg gccttgtagg
      301 tggtcttcat ctggcacttg tggttgcctc cgccttccag cttcaggtac atggtcacgt
      361 cgcccttcag cacgccgtcg ctggcggtga tcttctcggt gctaggctcc cagtccacgc
      421 tctggttctg catgataggt ccgtcggcag ggaagttcac gcctgtgaac ttgctcttgt
      481 ggtagaaggt gtttcctctc aggctgatgt gggcggacac gctagcgctt ccgccgtcct
      541 caaattccag gcttctctcc cagctcagtc cctcagggaa ggcctgcttg aagtagtcgg
      601 ggatttcctc agggtacttg gtgaacactc tgtgtccgta gcagaacacg tgagacacca
      661 ggtcgaaggc gaaaggcata ggtccgccct cggccatggt gactctcagg gtcatttcct
      721 ggtgtccctc gtaaggtctg ccggttccct cgccctcgat ggtaaactcg tggccgttca
      781 cagatccgtc catgtagtag cgcatcttca tctcaggctt gatcacagac accatcccgg
      841 gaggtccagg gttctcttcc acgtcgccgg cctgcttcag caggctgaag ttggtggctc
      901 cgcttcccct aggcttgtac agctcgtcca ttcccagggt gattccagcg gcggtcacaa
      961 attccagcag caccatgtgg tcgcgcttct cgttagggtc cttgctcagc ttgctctggg
     1021 tgctcaggt
//
