LOCUS       sequence-150060-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express flag-USP19 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: pRK-flag-USP19
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        222..240
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     222..239
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     253..272
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      312..321
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             321..344
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccatagt aagacaccgg
       61 gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc ccgtgccaag
      121 agtgacgtaa gtaccgccta tagagtctat aggcccaccc ccttggcttc gttagaacgc
      181 ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg acactataga
      241 ataacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa ctgcacctcg
      301 gttctaagct tgccgccatg gactacaagg acgacgatga caaggggtcg accatgtctg
      361 gcggggccag tgccacaggc ccaaggagag ggcccccagg actggaggac accactagta
      421 agaagaagca gaaggatcga gcaaaccagg agagcaagga tggagatcct aggaaagaga
      481 cagggtctcg atatgttgcc caggctggtc ttgaacctct ggcctcaggt gatccttctg
      541 cctcagcctc ccatgcagct gggatcacag gctcacgcca ccgtacccgg ctgttctttc
      601 cttcatcgtc agggtcagca tccactcctc aagaggagca gaccaaagag ggagcttgtg
      661 aagaccctca tgatctcttg gctactccca ctccagagtt gttgctcgat tggaggcaga
      721 gtgcagaaga ggtgattgtc aagcttcgtg tgggagtagg tcccctgcag ctggaggatg
      781 tagatgctgc tttcacagat acagactgtg tggtgcggtt
//
