LOCUS       sequence-150061-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express flag-USP19 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: pRK-flag-USP19
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..85
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        105..123
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     105..122
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
       61 tggcaactag aaggcacagt cgaggctgat cagcgagctc tagcatttag gtgacactat
      121 agaatagggc cctctagatg catgctcgag cggccgctta tctccagcga ctctgggata
      181 ccagaggata gaacacgttg agcacgaggg ccaccaaagc cgccacggtg cccaggacaa
      241 agtaccggag gcagccctca tctggtgtgg tagggccacg tggtaggggg cccacccagt
      301 cttggggccc ccagggaccc aggggcccag acccctcagg caccggctcc tcctcagcct
      361 ccagctcctg ccaaatccgg gaagcctggc tggcagcagc ctcagctgca gggcctaggt
      421 ctgggtggtg ctcagagtga cccgccctgg ggggcctctc cacaggagag ttccgccggc
      481 ggtagaagag tacataggca taacgcgtca caacctggct ctcgtctacc gttgtcactg
      541 tgctgtcatc aaacaagcgc cagcccacgt cactgcgctg actgctacga tcattgggca
      601 ggcgtgcaca ggcagtgtag tggccaccaa tcatgcctcc atagtggttg atgacagcat
      661 atagatcgta gctgggcagc tgctcctctt tctgaccaat gcagaacttg ctcaggtcca
      721 ggttcctaac agggaactcc accaagtcat tgatcttgtc acgccagata aaactacgaa
      781 aggagaagcg cttgagctgc acgatgagaa catttggcag gcgc
//
