LOCUS       sequence-150063-        4263 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express flag-USP19 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: pRK-flag-USP19
FEATURES             Location/Qualifiers
     source          1..4263
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        18..36
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             117..140
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(4141..4159)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4179..4196)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 tgtatcatac acatacgatt taggtgacac tatagaataa catccacttt gcctttctct
       61 ccacaggtgt ccactcccag gtccaactgc acctcggttc taagcttgcc gccatggact
      121 acaaggacga cgatgacaag gggtcgacca tgtctggcgg ggccagtgcc acaggcccaa
      181 ggagagggcc cccaggactg gaggacacca ctagtaagaa gaagcagaag gatcgagcaa
      241 accaggagag caaggatgga gatcctagga aagagacagg gtctcgatat gttgcccagg
      301 ctggtcttga acctctggcc tcaggtgatc cttctgcctc agcctcccat gcagctggga
      361 tcacaggctc acgccaccgt acccggctgt tctttccttc atcgtcaggg tcagcatcca
      421 ctcctcaaga ggagcagacc aaagagggag cttgtgaaga ccctcatgat ctcttggcta
      481 ctcccactcc agagttgttg ctcgattgga ggcagagtgc agaagaggtg attgtcaagc
      541 ttcgtgtggg agtaggtccc ctgcagctgg aggatgtaga tgctgctttc acagatacag
      601 actgtgtggt gcggtttgca ggtggtcagc agtggggtgg tgtcttctat gctgagataa
      661 aaagctcttg tgctaaagtg caaacccgca agggcagtct cctgcacctg acactgccca
      721 aaaaggtgcc tatgctcacg tggccctccc tcctggttga ggctgatgaa cagctttgca
      781 taccaccgct gaactcccaa acctgcctcc tgggctcaga ggagaattta gcccctttgg
      841 caggagagaa agcagtgcct cccgggaatg acccagtctc tccagccatg gtccggagca
      901 gaaaccctgg gaaagatgac tgtgccaagg aggagatggc agtggcagca gatgctgcaa
      961 ccttggtgga tgagcccgag tcgatggtga acctggcgtt tgtcaagaat gactcgtatg
     1021 agaagggccc ggattcagtg gtggtgcacg tgtacgtgaa ggagatctgc agggacacct
     1081 caagagtact tttccgtgag caggacttca cgctcatctt ccagaccagg gatggaaact
     1141 tcctgaggct gcacccgggc tgtgggcccc acaccacctt ccgttggcag gtgaagctca
     1201 ggaatctgat tgagccagag cagtgcacct tctgtttcac ggcttctcgc atcgacatct
     1261 gccttcgtaa gaggcagagt cagcgctggg ggggcctgga ggccccggct gcacgagtgg
     1321 gtggtgcaaa ggttgccgtg ccgacaggtc caacccctct ggattcaacc ccaccaggag
     1381 gtgctcccca ccccctgaca ggccaggagg aggcccgggc tgtggagaag gataaatcca
     1441 aggcacgatc tgaggacaca gggctagaca gtgtggcaac ccgcacaccc atggagcatg
     1501 taaccccaaa gccagagaca cacctggcct cgcccaagcc tacatgcatg gtgcctccca
     1561 tgccccacag cccagttagt ggagacagcg tggaggagga ggaagaggaa gagaagaagg
     1621 tgtgtctgcc aggcttcact ggccttgtca atttaggcaa cacctgcttc atgaacagcg
     1681 tcattcagtc tctgtccaac actcgggaac tccgggactt cttccatgac cgctcctttg
     1741 aggctgagat caactacaac aacccactag ggactggtgg gcgtctggcc attggctttg
     1801 ccgtgctgct tcgggcgctg tggaagggca cccaccatgc cttccagcct tccaagttga
     1861 aggccattgt ggcgagtaag gccagccagt tcacaggcta tgcacagcat gatgcccagg
     1921 agttcatggc tttcctgctg gatgggctgc acgaggacct gaatcgcatt cagaacaagc
     1981 cctacacaga gaccgtggat tcagatgggc ggcccgatga ggtggtagct gaggaagcat
     2041 ggcagcggca caagatgagg aatgactctt tcatcgtgga cctatttcag gggcagtaca
     2101 agtcgaagct ggtgtgccct gtgtgtgcca aggtctccat cacttttgac ccgtttcttt
     2161 atctgccggt gcccttgcca caaaagcaaa aggttctccc tgtcttttat tttgcccgag
     2221 agccccacag caagcccatc aagttcctgg tgagcgtcag caaggagaac tccactgcga
     2281 gcgaagtatt ggactccctc tctcagagtg ttcatgtgaa gcctgagaac ctgcgtttgg
     2341 cggaggtaat taagaatcgt tttcatcgtg tgttcctacc ctcccactca ctggacactg
     2401 tgtccccatc tgatacgctc ctctgctttg agctgctatc ctcagagttg gctaaggagc
     2461 gggtagtggt gctagaggtg caacagcgcc cccaggtgcc cagcgtcccc atctccaagt
     2521 gtgcagcctg ccagcggaag caacagtcgg aggatgaaaa gctgaagcgc tgtacccggt
     2581 gctaccgtgt gggctactgc aaccagctct gccagaaaac ccactggcct gaccacaagg
     2641 gcctctgccg acctgagaac attggctacc ccttcctggt cagtgtacct gcctcacgcc
     2701 tcacttatgc ccgcctcgct cagttgctag agggctatgc ccggtactct gtgagtgtat
     2761 tccagccacc ctttcagcca ggccgcatgg ccttggagtc tcagagccct ggctgcacca
     2821 cactgctctc cacaggttcc ctggaggctg gggacagcga gagagacccc attcagccac
     2881 ctgagctcca gctggtgacc cctatggctg agggggacac agggcttccc cgggtgtggg
     2941 cagcccctga ccggggtcct gtgcccagca ccagtggaat ttcttctgag atgctggcca
     3001 gtgggcccat tgaggttggc tccttgccag ctggcgagag ggtgtcccga cccgaagctg
     3061 ctgtgcctgg gtaccagcat ccaagtgaag ctatgaatgc ccacacaccc cagttcttca
     3121 tctataaaat tgattcatcc aaccgagagc agcggctaga ggacaaagga gacaccccct
     3181 tagaattagg tgacgactgt agcctggctc tcgtctggcg gaacaatgag cgcttgcagg
     3241 agtttgtgtt ggtagcctcc aaggagctgg aatgtgctga ggatccaggc tctgccggtg
     3301 aggctgcccg ggccggccac ttcaccctgg accagtgcct caacctcttc acacggcctg
     3361 aggtgctggc acccgaggag gcctggtact gcccacagtg caaacagcac cgtgaggcct
     3421 ccaagcagct gttgctatgg cgcctgccaa atgttctcat cgtgcagctc aagcgcttct
     3481 cctttcgtag ttttatctgg cgtgacaaga tcaatgactt ggtggagttc cctgttagga
     3541 acctggacct gagcaagttc tgcattggtc agaaagagga gcagctgccc agctacgatc
     3601 tatatgctgt catcaaccac tatggaggca tgattggtgg ccactacact gcctgtgcac
     3661 gcctgcccaa tgatcgtagc agtcagcgca gtgacgtggg ctggcgcttg tttgatgaca
     3721 gcacagtgac aacggtagac gagagccagg ttgtgacgcg ttatgcctat gtactcttct
     3781 accgccggcg gaactctcct gtggagaggc cccccagggc gggtcactct gagcaccacc
     3841 cagacctagg ccctgcagct gaggctgctg ccagccaggc ttcccggatt tggcaggagc
     3901 tggaggctga ggaggagccg gtgcctgagg ggtctgggcc cctgggtccc tgggggcccc
     3961 aagactgggt gggcccccta ccacgtggcc ctaccacacc agatgagggc tgcctccggt
     4021 actttgtcct gggcaccgtg gcggctttgg tggccctcgt gctcaacgtg ttctatcctc
     4081 tggtatccca gagtcgctgg agataagcgg ccgctcgagc atgcatctag agggccctat
     4141 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     4201 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     4261 cac
//
