LOCUS       sequence-150072-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression flag-USP19 494-1216 (USP) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: pRK-flag-USP19 494-1216 (USP)
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        221..239
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     221..238
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     252..271
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      311..320
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             320..343
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
       61 accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga
      121 gtgacgtaag taccgcctat agagtctata ggcccacccc cttggcttcg ttagaacgcg
      181 gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa
      241 taacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac tgcacctcgg
      301 ttctaagctt gccgccatgg actacaagga cgacgatgac aaggggtcga ccccaggctt
      361 cactggcctt gtcaatttag gcaacacctg cttcatgaac agcgtcattc agtctctgtc
      421 caacactcgg gaactccggg acttcttcca tgaccgctcc tttgaggctg agatcaacta
      481 caacaaccca ctagggactg gtgggcgtct ggccattggc tttgccgtgc tgcttcgggc
      541 gctgtggaag ggcacccacc atgccttcca gccttccaag ttgaaggcca ttgtggcgag
      601 taaggccagc cagttcacag gctatgcaca gcatgatgcc caggagttca tggctttcct
      661 gctggatggg ctgcacgagg acctgaatcg cattcagaac aagccctaca cagagaccgt
      721 ggattcagat gggcggcccg atgaggtggt agctgaggaa gcatggcagc ggcacaagat
      781 gaggaatgac tctttcatcg tggacctatt tcagg
//
