LOCUS       sequence-150073-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression flag-USP19 494-1216 (USP) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: pRK-flag-USP19 494-1216 (USP)
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..84
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        104..122
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     104..121
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gtgggagtgg caccttcagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
       61 ggcaactaga aggcacagtc gaggctgatc agcgagctct agcatttagg tgacactata
      121 gaatagggcc ctctagatgc atgctcgagc ggccgcttac cggaggcagc cctcatctgg
      181 tgtggtaggg ccacgtggta gggggcccac ccagtcttgg ggcccccagg gacccagggg
      241 cccagacccc tcaggcaccg gctcctcctc agcctccagc tcctgccaaa tccgggaagc
      301 ctggctggca gcagcctcag ctgcagggcc taggtctggg tggtgctcag agtgacctgc
      361 cctgggaggc ctctccactc aagagttccg ccggcggtag aagagtacat aggcataacg
      421 cgtcacaacc tggctctcgt ctaccgttgt cactgtgctg tcatcaaaca agcgccagcc
      481 cacgtcactg cgctgactgc tacgatcatt gggcaggcgt gcacaggcag tgtagtggcc
      541 accaatcatg cctccatagt ggttgatgac agcatataga tcgtagctgg gcagctgctc
      601 ctctttctga ccaatgcaga acttgctcag gtccaggttc ctaacaggga actccaccaa
      661 gtcattgatc ttgtcacgcc agataaaact acgaaaggag aagcgcttga gctgcacgat
      721 gagaacattt ggcaggcgcc atagcaacag ctgcttgga
//
