LOCUS       sequence-150077-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c-FL3
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..564
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="TLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFTLR
                     EVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRRLT
                     VEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTLCG
                     LDTALYDGTASDGEQALYMSMPCP"
     terminator      570..767
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(872..891)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(873..891)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actcttgacg acacggctta ccggtaccgc accagtgtcc cgggggacgc cgaggccatc
       61 gaggcactgg atgggtcctt caccaccgac accgtcttcc gcgtcaccgc caccggggac
      121 ggcttcaccc tgcgggaggt gccggtggac ccgcccctga ccaaggtgtt ccccgacgac
      181 gaatcggacg acgaatcgga cgacggggag gacggcgacc cggactcccg gacgttcgtc
      241 gcgtacgggg acgacggcga cctggcgggc ttcgtggtcg tctcgtactc cggctggaac
      301 cgccggctga ccgtcgagga catcgaggtc gccccggagc accgggggca cggggtcggg
      361 cgcgcgttga tggggctcgc gacggagttc gcccgcgagc ggggcgccgg gcacctctgg
      421 ctggaggtca ccaacgtcaa cgcaccggcg atccacgcgt accggcggat ggggttcacc
      481 ctctgcggcc tggacaccgc cctgtacgac ggcaccgcct cggacggcga gcaggcgctc
      541 tacatgagca tgccctgccc ctaatcagta ctgacaataa aaagattctt gttttcaaga
      601 acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg
      661 tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga
      721 aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat
      781 actaacgccg ccatccagtg ttaaacgagc tcgaattcat cgatgatatc agatccacta
      841 gtggcctatg cggccgcgga tctgccggtc tccctatagt gagtcgtatt aatttcgata
      901 agccaggtta acctgcatta atgaatcggc caacgcgcgg gg
//
