LOCUS       sequence-150093-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Small fragment of split HRP targeted for expression in the lumen of 
            the endoplasmic reticulum (ER). CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: pDisplay sHRPb-KDEL
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     153..212
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             237..278
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             609..638
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tagcgagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccactagta acggccgcca
      121 gtgtgctgga attcggcttg gggatatcca ccatggagac agacacactc ctgctatggg
      181 tactgctgct ctgggttcca ggttccactg gtgacggggc ccagccggcc agatctggca
      241 agcccatccc caaccccctg ctgggcctgg acagcaccaa cttgtctgca ctggttgact
      301 ttgacctgcg caccccaacc atcttcgaca ataagtatta cgtcaatctg gaggaacaga
      361 agggcctgat tcagtccgac caggagctgt tcagttcccc agatgctacc gacactattc
      421 ctcttgtgcg cagcttcgct aatagcacac aaaccttttt caatgctttc gtggaagcta
      481 tggatcggat gggaaatata acgcccctga cgggtacgca gggacagata agacggaact
      541 gtagggtcgt gaacagcaac tctggcagcg gcaaggacga gctgtaataa tgtggcgcgc
      601 cagtcgacga acaaaaactc atctcagaag aggatctgaa tgctgtgggc caggacacgc
      661 aggaggtcat cgtggtgcca cactccttgc cctttaaggt ggtggtgatc tcagccatcc
      721 tggccctggt ggtgctcacc atcatctccc ttatcatcct catcatgc
//
